General
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Antigen
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KIT Ligand (KITLG) Antibodies
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Reactivity
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Human
Alternatives
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Host
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Rabbit
Alternatives
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Clonality
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Conjugate
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This KIT Ligand antibody is un-conjugated
Alternatives
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Application
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Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
Alternatives
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Supplier
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Log in to see
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Purification
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Antigen affinity purified polyclol antibody
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Sterility
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Sterile filtered
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Immunogen
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recombint hSCF (human SCF)
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Isotype
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IgG
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Antigen
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Alternative Name
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Stem Cell Factor (KITLG Antibody Abstract)
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Background
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Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSCF (human SCF). Anti-Human SCF specific antibody was purified by affinity chromatography employing immobilized hSCF matrix.
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Pathways
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RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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Application Notes
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To yield one-half maximal inhibition [ND₅₀] of the biological activity of hSCF (10.0 ng/µl), a concentration of 0.64 - 0.96 µg/µl of this antibody is required. To detect hSCF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. To detect hSCF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human SCF (PK-AB815-63120B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect hSCF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human SCF (PK-AB815-63120B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF. To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Restrictions
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For Research Use only
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Format
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Lyophilized
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Concentration
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To yield one-half maximal inhibition [ND₅₀] of the biological activity of hSCF (10.0 ng/µl), a concentration of 0.64 - 0.96 µg/µl of this antibody is required.
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Buffer
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A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
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Handling Advice
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The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
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Storage
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-20 °C
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Expiry Date
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24 months
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