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|Antigen||Tuberous Sclerosis 1 (TSC1) Antibodies|
|Epitope||Middle Region, both Isoforms Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TSC1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-TSC1 AntibodyTarget Details TSC1 Application Details Handling Images
|Specificity||This antibody detects TSC1 / Hamartin at Center.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Peptide affinity chromatography|
|Immunogen||TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.|
Target Details TSC1Product Details anti-TSC1 Antibody Application Details Handling Images back to top
|Alternative Name||TSC1 / Hamartin (TSC1 Antibody Abstract)|
|Background||Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.Synonyms: KIAA0243, TSC, Tuberous sclerosis 1 protein|
|Pathways||RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation|
Application DetailsProduct Details anti-TSC1 Antibody Target Details TSC1 Handling Images back to top
ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TSC1 Antibody Target Details TSC1 Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|