Goat anti-Human IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN101645

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(c), The anti-Human IgG F(c) Antibody detects the Fc constant subdomain of the heavy chain subunit.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Human IgG F(c) fragment

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Goat Anti-Human IgG F(c) Antibody is suitable for immunoassays where specificity to the immunoglobulin Fc region is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.

ELISA Dilution: 1:20,000

Western Blot Dilution: 1:10,000 - 1:50,000

Comment:

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat Anti Human IgG Fc Antibody, Goat Anti-Human IgG F(c) Antibody, Goat Anti-Human IgG Fc Fragment Antibody

Background: Anti-Human IgG F(c) generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.