Goat anti-Mouse IgG (Fc Region) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN101856

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(c)

Cross-Reactivity: Mouse (Murine)

Characteristics: Anti-Mouse IgG F(c) Antibody generated in goat detects specifically mouse IgG F(c). Anti-Mouse IgG F(c) antibody is ideal for investigators involved in Serum Protein Component research. Anti-Mouse IgG F(c) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Mouse IgG F(c) Antibody has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for Anti-Mouse IgG F(c) Antibody.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Mouse IgG F(c) Antibody, Gt-a-Mouse Biotin conjugated, Mouse IgG F(c) Antibody in goat, Goat IgG F(c) Biotin conjugated Secondary Antibody.

Background: Anti-Mouse IgG F(c) Antibody generated in goat detects specifically mouse IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Mouse IgG F(c) Biotin conjugation antibody is ideal for investigators involved in Serum Protein Component research, Cancer, Immunology, and Microbiology research.