Mouse anti-Human IgA + IgD + IgE + IgG + IgM Antibody

Catalog No. ABIN1099692

Product Details

Target: IgA + IgD + IgE + IgG + IgM

Reactivity: Human

Host: Mouse

Clonality: Polyclonal

Application: Immunofluorescence (IF), ELISA

Characteristics: The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.

Monoclonal antibody concentration in the solution is 1.0 mg/mL.

Physical form: Delipidated, heat inactivated, lyophilized stable ascites.

Purification: Ascites

Immunogen: Highly purified monoclonal IgG subclasses isolated from pooled human serum.

Application Details

Application Notes: The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA + IgD + IgE + IgG + IgM

Background: To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.