Rabbit anti-Human IgG (Chain gamma) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN117260

Product Details

Target: IgG

Binding Specificity: Chain gamma

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG gamma chains.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other phosphatase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 g of Human IgG in astandard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutesat room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA IgG and Protease free) and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin G