Goat anti-Mouse IgG, IgM (Heavy & Light Chain) Antibody - Preadsorbed
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- Target See all IgG, IgM products
- IgG, IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- IgG + IgM (H&L)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG and IgM whole molecules
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:2,000 - 1:10,000
Application Note: Anti-Mouse IgG + IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:100,000
Western Blot Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG, IgM
- Alternative Name
- IgG + IgM (IgG, IgM Products)
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti Mouse IgG + IgM Antibody Pre-Adsorbed, Goat Anti-Mouse IgG/IgM Antibody
Background: Anti-Mouse IgG + IgM Antibody generated in Goat detects reactivity to Mouse IgG and IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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