Goat anti-Mouse IgG, IgM (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed
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- Target See all IgG, IgM products
- IgG, IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Texas Red (TR)
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Application
- Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
- Specificity
- IgG + IgM (H&L)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG and IgM whole molecules
- Isotype
- IgG
- Labeling Ratio
- 3.7
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Texas Red™ is a registered trademark of Molecular Probes Inc.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG, IgM
- Alternative Name
- IgG + IgM (IgG, IgM Products)
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti-Mouse IgG + IgM Antibody Texas Red™ Conjugated Pre-Adsorbed, Goat Anti-Mouse IgG/IgM Antibody Texas Red™ Conjugated
Background: Anti-Mouse IgG + IgM Texas Red Antibody generated in Goat detects reactivity to Mouse IgG and IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Texas Red Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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