Goat anti-Rabbit IgG Antibody (Agarose Beads)

Catalog No. ABIN233744

Product Details

Target: IgG

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Agarose Beads

Application: Immunoprecipitation (IP)

Characteristics: Anti Rabbit IgG Secondary Antibody Agarose can be used for immunoaffinity chromatography of polyclonal or monoclonal rabbit IgG antibodies. Once bound, non-specific antibodies are removed by copious washing followed by elution of specific antibody at low pH.

Purification: This product is an IgG fraction antibody coupled to activated agarose.  Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure Rabbit IgG.

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti Rabbit IgG Secondary Antibody Agarose can be used for immunoaffinity chromatography of polyclonal or monoclonal rabbit IgG antibodies. Once bound, non-specific antibodies are removed by copious washing followed by elution of specific antibody at low pH .

Immunoprecipitation Dilution: User Optimized

Restrictions: For Research Use only

Handling

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Sensitive to light.
Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat anti RABBIT IgG Secondary Antibody Agarose conjugate

Background: Anti-Rabbit IgG Agarose Conjugate generated in goat detects rabbit Immunoglobulin G. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.