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Pig anti-Human IgM (Fc Region) Antibody (FITC)

ELISA, IHC, IF, ICC, IHC (fro), ID Polyclonal FITC

Catalog No. ABIN2623670

Datasheet as PDF

Target See all IgM products

IgM
Binding Specificity
132

110

86

41

16

8

7

1

1
Fc Region
Reactivity
185

129

65

37

30

23

22

19

14

13

13

13

13

12

11

4

2
Human
Host
312

176

73

15

13

5

4

1
Pig
Clonality
484

78
Polyclonal
Conjugate
109

76

76

55

32

25

24

15

12

11

11

11

10

9

9

9

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1
FITC
Application
312

236

228

187

143

128

64

62

47

47

46

42

38

28

20

18

13

6

5

5

3

3

2

2

1

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID)

Specificity

Human IgM, Fc specific.

Purification

Purified IgG

Immunogen

Purified normal IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein
Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP

Goat anti-Human IgM (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgM antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgM antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgM (Heavy Chain) antibody (FITC)
ELISA, FACS, IHC Polyclonal IgG FITC

Goat anti-Human IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC

Goat anti-Human IgM (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)

Goat anti-Human IgM antibody (HRP) - Preadsorbed
ELISA, FLISA Polyclonal IgG HRP

Goat anti-Human IgM (Chain mu) antibody (DyLight 594)
FACS, IF DyLight 594

Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Application Notes

Approved: ELISA, ICC, ID, IF, IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunits of the IgM molecule which expresses strict isotypic specificity. The conjugate does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Distilled water

Concentration

Lot specific

Buffer

Lyophilized from PBS,  pH 7.2. No preservative added.

Preservative

Without preservative

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

IgM

Abstract

IgM Products

Target Type

Antibody