Mouse anti-Armenian Hamster kappa Light Chain Antibody (Biotin)

Catalog No. ABIN2689606

Product Details

Target: kappa Light Chain

Reactivity: Rat, Golden Syrian Hamster, Armenian Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The RG7/7.6 antibody reacts strongly with rat Igs bearing - light chain of the Igk-1[a]allotype (e.g., AO, AUG, BN, BUF, LEW, LOU, PVG, WAG, WF), weakly with Igk-1[b] light chains (e.g., ACI, COP, DA, F344, SHR), and it does not react with λ light chain nor heavy chains. At the time that this antibody was characterized, the Igk-1[a] allotype was designated - 1b, and Igk-1[b] was -1a, the current nomenclature was standardized at the Fourth International Workshop on Allogenic Systems in the Rat. The RG7/7.6 antibody also strongly cross-reacts with Syrian and Armenian hamster Ig - light chain.

BD Pharmingen™ Biotin Mouse Anti-Rat and Anti-Hamster Ig, κ Light Chain - Biotin - Clone RG7/7.6 - Isotype Mouse IgG2a, κ - Reactivity Rat, Ar Ham, Syr Ham - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Pooled Rat IgG

Clone: RG7-7-6

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Butcher: "A list of monoclonal antibodies specific for alloantigens of the rat." in: Journal of immunogenetics, Vol. 14, Issue 2-3, pp. 163-76, (1988) (PubMed).

Springer, Bhattacharya, Cardoza, Sanchez-Madrid: "Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies." in: Hybridoma, Vol. 1, Issue 3, pp. 257-73, (1984) (PubMed).

Target Details

Target: kappa Light Chain

Alternative Name: Ig, kappa Light Chain (kappa Light Chain Products)