Goat anti-Human IgG (Fc Region) Antibody (Biotin)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Fc Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab')2 or Bovine, Horse, Mouse and Rat Serum Proteins
- Purification
- Immunoaffinity purified
- Immunogen
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Human IgG Fc fragment.
Type of Immunogen: Purified protein - Isotype
- IgG
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- Application Notes
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Approved: ELISA (1:20000 - 1:100000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- RT,4 °C
- Storage Comment
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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