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Goat anti-Mouse IgM (Heavy Chain) Antibody (FITC)

FACS, IF Polyclonal IgG FITC F(ab')2 fragment
Catalog No. ABIN301388
  • Target See all IgM products
    IgM
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 162
    • 123
    • 87
    • 57
    • 26
    • 10
    • 7
    • 3
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 225
    • 156
    • 88
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    Mouse
    Host
    • 365
    • 231
    • 85
    • 18
    • 14
    • 5
    • 4
    • 1
    • 1
    Goat
    Clonality
    • 534
    • 88
    Polyclonal
    Conjugate
    • 121
    • 86
    • 84
    • 65
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    FITC
    Application
    • 375
    • 281
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 64
    • 62
    • 53
    • 52
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Specificity
    Anti- Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti- Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes
    Purification
    Immunoaffinity purified
    Immunogen
    Mouse IgM mu chain.

    Type of Immunogen: Purified protein
    Isotype
    IgG
  • Application Notes
    Approved: Flo (1:2000 - 1:10000), IF (1:500 - 1:2500)

    Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Comment

    Target Species of Antibody: Mouse

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    deionized water
    Concentration
    Lot specific
    Buffer
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Avoid freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
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