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Mouse anti-Rat IgM Antibody (Biotin)

FACS, IF, EIA Monoclonal IgG1 Biotin
Catalog No. ABIN371243
  • Target See all IgM products
    IgM
    Reactivity
    • 225
    • 156
    • 88
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    Rat
    Host
    • 365
    • 231
    • 85
    • 18
    • 14
    • 5
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 534
    • 88
    Monoclonal
    Conjugate
    • 121
    • 86
    • 84
    • 65
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Biotin
    Application
    • 375
    • 281
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 64
    • 62
    • 53
    • 52
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Isotype
    IgG1
  • Application Notes
    Flow Cytometry (< / = 0.3 μg/10^6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. ELISA. Second step reagent for rat IgM Monoclonal Antibodies. Surface IgM Detection. B Cell Stimulation.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    PBS containing 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -28 °C for longer.
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background
    IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.Synonyms: Rat Immunoglobulin M
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