Goat anti-Rat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN398396

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Characteristics: This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rat IgG heavy and light chains.

Purification: Immunoaffinity chromatography

Immunogen: Full-length rat IgG (purified)

Isotype: IgG

Application Details

Application Notes: Antibodies-Online Goat Anti-Rat IgG (H&L) [HRP] is suitable for immunoblotting (Western and Dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:5,000-1:50,000
Western blot: 1:1,000-1:5,000Other applications: user-optimized.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Handling Advice: Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below. Avoid repeated freezing and thawing cycles.

References

Deckert, Kretz, Habbout, Raghay, Labbé, Abello, Desrumaux, Gautier, Lemaire-Ewing, Maquart, Le Guern, Masson, Steinmetz, Lagrost: "Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency." in: The American journal of pathology, Vol. 183, Issue 3, pp. 975-86, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody