Goat anti-Human IgG + IgA + IgM + IgD (Fab Region) Antibody - Preadsorbed

Catalog No. ABIN457998

Product Details

Target: IgG + IgA + IgM + IgD

Binding Specificity: Fab Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP)

Specificity: The defined antibody specificity is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibody to common determinants and to class specific determinants and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA, IgM and IgD (if present in precipitable amounts) precipitin lines are obtained.

Characteristics: Ig fraction of polyclonal goat antiserum to human immunoglobulins IgG, IgA, IgM and IgD, heavy and light chains
Antibody titre: Precipitin titre not less than 1:64 when tested against normal human serum in agar immunodiffusion block titration.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Highly purified IgG, and pools of homogenous IgA, IgM and IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This antiserum is intended to detect and identify polyclonal immunoglobulins, purified monoclonal immunoglobulins in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electro-phoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/mL in the presence of normal levels of immunoglobulins. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG + IgA + IgM + IgD