Rabbit anti-Monkey IgA, IgG, IgM (Fab Region) Antibody (FITC) - Preadsorbed

Catalog No. ABIN458314

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Fab Region

Reactivity: Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Specificity: The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). .

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to monkey IgG, IgA and IgM, heavy and light chains
Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.6.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates, to demonstrate immunoglobulins or specific antibodies in cells and tissues, to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably use d the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM (IgA, IgG, IgM Products)

Target Type: Antibody