Rabbit anti-Rat IgA, IgG, IgM (Fab Region), (Light Chain) Antibody (FITC)

Catalog No. ABIN458729

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Fab Region, Light Chain

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Specificity: The reactivity of the antiserum is directed to all known isotypes of the rat immunoglobulin system (classes, subclasses and light chain types) including antibodies to common determinants, to class and subclass specific determinants, and to the surface determinants of the common Fab portion as tested by immunoelectrophoresis and double radial immuno-diffusion.

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to rat immunoglobulins, heavy and light chains
Fluorochrome/IgG protein molar ratio (F/P) is approxi-mately 1.7.

Purification: No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Purified polyclonal rat IgG, including all subclasses, and pools of homogenous immuno-globulins. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: To identify rat immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM (IgA, IgG, IgM Products)

Target Type: Antibody