Rabbit anti-Mouse Immunoglobulin kappa Chain Complex (Igk) (Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN5608148

Product Details

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Binding Specificity: Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Mouse (Murine)

Characteristics: Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Mouse kappa light chain was produced by repeated immunization with Mouse kappa light chain in rabbit.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

ELISA Dilution: 1:10,000 - 50,000

Western Blot Dilution: 1:2,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Alternative Name: kappa ) (Igk Products)

Background:

Synonyms: Rabbit Anti-Mouse κ (kappa chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse kappa Antibody HRP Conjugation

Background: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to peroxidase.