Rabbit anti-Mouse IgG2a (Chain gamma 2a), (Heavy Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN616347

Product Details

Target: IgG2a

Binding Specificity: Chain gamma 2a, Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Characteristics: Molar Ratio: 3.6 moles FITC per mole of Rabbit IgG.

Purification: Preadsorption: Solid phase adsorption(s) against a panel of imobilized mouse and human serum proteins resulting in a conjugated antibody designed for Flow cytometry. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Mouse or Human heavy or light chain isotypes.

Immunogen: Mouse IgG2a heavy chain.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Absorption / Emission: 495 nm / 528 nm

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and Protease free) as stabilzer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: IgG2a

Abstract: IgG2a Products

Target Type: Antibody

Background: Synonyms: Mouse Immunoglobulin 2a