Goat anti-Human IgA (Chain alpha) Antibody (HRP)

Catalog No. ABIN619562

Quick Overview for Goat anti-Human IgA (Chain alpha) Antibody (HRP) (ABIN619562)

Target: IgA

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Chain alpha

Reactivity: Human

Host: Goat

Cross-Reactivity: Sheep (Ovine), Mouse (Murine), Rabbit

Predicted Reactivity: human IgA heavy chain (alpha)

Characteristics: Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) in immunological assays. It is especially adsorbed against bovine/mouse/rabbit serum and affinity purified.

Purification: Affinity purification

Immunogen: purified human IgA, heavy (alpha chain)

Application Details

Application Notes: optimal working dilution should be determined by the investigator

Comment:

No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgA

Target Type: Antibody