Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 800) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Goat
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- DyLight 800
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Purpose
- Donkey anti-Goat IgG Antibody DyLight™ 800 Conjugated Pre-Adsorbed
- Specificity
- This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
- Characteristics
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Synonyms: Donkey anti-Goat IgG Antibody DyLight™ 800 Conjugated Pre-Adsorbed, Donkey anti-Goat IgG DyLight™ 800 Conjugated Antibody
Background: Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.
- Immunogen
- Immunogen: Goat IgG, whole molecule
- Isotype
- IgG
- Labeling Ratio
- 2.7
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- Application Notes
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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An inhibitor of proteasome β2 sites sensitizes myeloma cells to immunoproteasome inhibitors." in: Blood advances, Vol. 2, Issue 19, pp. 2443-2451, (2018) (PubMed).
: "
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An inhibitor of proteasome β2 sites sensitizes myeloma cells to immunoproteasome inhibitors." in: Blood advances, Vol. 2, Issue 19, pp. 2443-2451, (2018) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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