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Goat anti-Mouse IgM (Chain mu) Antibody (HRP) - Preadsorbed

ELISA, WB Polyclonal HRP F(ab')2 fragment

Catalog No. ABIN965168

Datasheet as PDF

Target See all IgM products

IgM

Fragment

F(ab')2 fragment
Binding Specificity
140

117

87

41

17

8

7

1

1
Chain mu
Reactivity
189

129

65

39

32

26

22

21

16

15

15

14

13

13

12

4

2
Mouse
Host
320

188

73

15

14

5

4

1

1
Goat
Clonality
495

78
Polyclonal
Conjugate
120

76

76

55

36

32

24

15

12

11

11

11

10

9

9

9

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1
HRP
Application
334

250

236

209

143

139

64

62

47

47

46

42

38

29

20

18

13

6

5

5

3

3

2

2

1

1

1

1

1

1
ELISA, Western Blotting (WB)

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum.

Characteristics

F(ab')2 Anti-Mouse IgM mu heavy chain generated in goat detects specifically Mouse IgM mu heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification

Preadsorption: Solid phase absorption

Immunogen

F(ab')2 Anti-Mouse IgM mu heavy chain was produced by repeated immunization with Mouse IgM mu heavy chain in goat.
Immunogen Type: Native Protein
Goat anti-Mouse IgM (Whole Molecule) antibody (HRP)
ELISA, WB, DB Polyclonal IgM HRP

Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)
ELISA, IHC, FACS, WB Polyclonal IgG Biotin

Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Mouse IgM antibody (APC) - Preadsorbed
ELISA, FACS Polyclonal IgG APC

Goat anti-Mouse IgM antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Goat anti-Mouse IgM (Heavy Chain) antibody
ELISA, IHC, FACS, WB Polyclonal IgG unconjugated

Goat anti-Mouse IgM (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Mouse IgM (Heavy Chain) antibody (beta-Gal)
ELISA, IHC Polyclonal IgG beta-Gal

Application Notes

F(ab') 2 Anti-Mouse IgM mu heavy chain is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration

2.2 mg/mL

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

Gentamicin sulfate

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage

4 °C

Expiry Date

12 months
Target

IgM

Abstract

IgM Products

Target Type

Antibody

Background

Synonyms: F(ab')2 Mouse Antibody, Gt-a-Mouse IgM mu chain, Mouse IgM mu chain Antibody in Goat, F(ab')2 Mouse Secondary Antibody