Mouse anti-Human IgG (Fc Region) Antibody

Catalog No. ABIN966351

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Specificity: Chrompure Human IgG, Fc Fragment

Characteristics: Monoclonal Anti-Human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1:200- 1:1,000
ELISA: Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References

Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Gene ID: 2209