anti-Rat (Rattus) COL2A1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended COL2A1 Antibody (supplied by: Log in to see )

Antigen
Collagen, Type II, alpha 1 (COL2A1) Antibodies
  • ANFH
  • AOM
  • COL11A3
  • SEDC
  • Col2
  • Col2a
  • Col2a-1
  • Del1
  • Dmm
  • Lpk
  • M100856
  • Rgsc856
  • CG2A1A
  • COLLII
  • col2a1a
  • CB11
  • col2a1
  • coll2a1
  • fb38c06
  • fc10c01
  • wu:fb38c06
  • wu:fc10c01
  • collagen type II alpha 1 chain
  • collagen, type II, alpha 1
  • collagen, type II, alpha 1 L homeolog
  • collagen, type II, alpha 1 S homeolog
  • collagen, type II, alpha 1a
  • COL2A1
  • Col2a1
  • col2a1
  • col2a1.L
  • col2a1.S
  • col2a1a
Epitope
Chain alpha 1
18
15
14
10
4
4
3
2
2
2
2
2
1
1
1
Reactivity
Cow (Bovine), Human, Rabbit, Rat (Rattus)
55
37
32
7
3
3
3
2
2
Host
Mouse
68
6
1
1
Clonality
Monoclonal
Conjugate
This COL2A1 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
22
19
17
17
15
15
13
9
6
5
3
1
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Catalog No. ABIN567566
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN265366 IF IHC (p) WB Rabbit Chain alpha 1 Log in to see Polyclonal 0
1 ABIN1450180 EIA IHC (fro) IHC (p) IP WB Rabbit Chain alpha 1 Log in to see Polyclonal 0
1 ABIN116552 EIA IHC (fro) IHC (p) IP WB Rabbit Chain alpha 1 Log in to see Polyclonal 0
1 ABIN2487215 EIA IHC (fro) IF IHC (p) RIA Rabbit Chain alpha 1 Log in to see Polyclonal 0

General

Antigen Collagen, Type II, alpha 1 (COL2A1) Antibodies
Epitope Chain alpha 1
(18), (15), (14), (10), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Human, Rabbit, Rat (Rattus)
(55), (37), (32), (7), (3), (3), (3), (2), (2)
Host Mouse
(68), (6), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This COL2A1 antibody is un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (22), (19), (17), (17), (15), (15), (13), (9), (6), (5), (3), (1)
Pubmed 7 references available
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Product Details anti-COL2A1 Antibody

Target Details COL2A1 Application Details Handling References for anti-COL2A1 antibody (ABIN567566) Images
Specificity The antibody specifically reacts with Collagen type II from human costal cartilage.
Cross-Reactivity (Details) Species reactivity (tested):Human, Rabbit, Bovine, and Rat.
Purification Affinity Chromatography on Protein A.
Clone II-4C11
Isotype IgG1

Target Details COL2A1

Product Details anti-COL2A1 Antibody Application Details Handling References for anti-COL2A1 antibody (ABIN567566) Images back to top
Antigen
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.Synonyms: Alpha-1 type II collagen, COL2A1
Gene ID 1280
Pathways Sensory Perception of Sound, Growth Factor Binding

Application Details

Product Details anti-COL2A1 Antibody Target Details COL2A1 Handling References for anti-COL2A1 antibody (ABIN567566) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-COL2A1 Antibody Target Details COL2A1 Application Details References for anti-COL2A1 antibody (ABIN567566) Images back to top
Concentration 0.5 mg/mL
Buffer 0.1 M Na-Phosphate buffer, pH 7.0, 0.5 % BSA (protease-free)

References for anti-COL2A1 antibody (ABIN567566)

Product Details anti-COL2A1 Antibody Target Details COL2A1 Application Details Handling Images back to top
Product cited in:

Guo, Lu, Ji, Ma, Dong, Tu, Guo, Qiu, Wu, Zhang: "Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability." in: Ultrasonics, Vol. 58, pp. 43-52, 2015

Kleinhans, Mohan, Vacun, Schwarz, Haller, Sun, Kahlig, Kluger, Finne-Wistrand, Walles, Hansmann: "A perfusion bioreactor system efficiently generates cell-loaded bone substitute materials for addressing critical size bone defects." in: Biotechnology journal, 2015

Sieker, Kunz, Weißenberger, Gilbert, Frey, Rudert, Steinert: "Direct bone morphogenetic protein 2 and Indian hedgehog gene transfer for articular cartilage repair using bone marrow coagulates." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 23, Issue 3, pp. 433-42, 2015

Bleil, Sieper, Maier, Schlichting, Hempfing, Syrbe, Appel: "Cartilage in facet joints of patients with ankylosing spondylitis (AS) shows signs of cartilage degeneration rather than chondrocyte hypertrophy: implications for joint remodeling in AS." in: Arthritis research & therapy, Vol. 17, pp. 170, 2015

Yan, Silva-Correia, Oliveira, Vilela, Pereira, Sousa, Mano, Oliveira, Oliveira, Reis: "Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance." in: Acta biomaterialia, Vol. 12, pp. 227-41, 2014

Background publications

Kumagai, Sarkar, Uhthoff, Okawara, Ooshima: "Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion." in: Journal of anatomy, Vol. 185 ( Pt 2), pp. 279-84, 1994

Burgeson, Hollister: "Collagen heterogeneity in human cartilage: identification of several new collagen chains." in: Biochemical and biophysical research communications, Vol. 87, Issue 4, pp. 1124-31, 1979

Images

Product Details anti-COL2A1 Antibody Target Details COL2A1 Application Details Handling References for anti-COL2A1 antibody (ABIN567566) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Collagen, Type II, alpha 1 (COL2A1) (Chain alpha 1) antibody (ABIN567566) Immunohistochemistry on human trachea paraffin sections using Collagen type II antibo...