anti-Horse (Equine) Nanog Homeobox antibody for Western Blotting

Recommended Nanog Homeobox Antibody (supplied by: Log in to see )

Antigen
Nanog Homeobox (NANOG) Antibodies
  • 2410002E02Rik
  • ENK
  • ecat4
  • NANOG
  • hacp
  • wu:fd19e04
  • wu:fd20a07
  • zgc:193933
  • Nanog homeobox
  • nanog homeobox
  • Nanog
  • NANOG
  • nanog
Epitope
Middle Region
19
18
18
16
15
15
15
13
12
9
9
9
8
7
6
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
307
83
49
7
6
5
3
3
3
1
1
Host
Rabbit
232
64
48
2
Clonality
Polyclonal
Conjugate
Un-conjugated
17
16
16
15
14
13
3
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
261
185
98
82
39
36
36
26
23
17
9
9
4
3
2
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN2777175
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4.8440056 ABIN2783183 WB Rabbit N-Term Log in to see Polyclonal 1

General

Antigen Nanog Homeobox (NANOG) Antibodies
Epitope Middle Region
(19), (18), (18), (16), (15), (15), (15), (13), (12), (9), (9), (9), (8), (7), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(307), (83), (49), (7), (6), (5), (3), (3), (3), (1), (1)
Host Rabbit
(232), (64), (48), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(17), (16), (16), (15), (14), (13), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(261), (185), (98), (82), (39), (36), (36), (26), (23), (17), (9), (9), (4), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN980475
Sequence SYKQVKTWFQ NQRMKSKRWQ KNNWPKNSNG VTQKASAPTY PSLYSSYHQG
Predicted Reactivity Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 86%, Rat: 92%, Sheep: 86%
Characteristics This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NANOG

Target Details

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Antigen
Alternative Name NANOG (NANOG Antibody Abstract)
Background NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305
Molecular Weight 34 kDa
Gene ID 79923
NCBI Accession NM_024865, NP_079141
UniProt Q6NSW7
Pathways Stem Cell Maintenance

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 305 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Kallas, Pook, Maimets, Zimmermann, Maimets: "Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells." in: PLoS ONE, Vol. 6, Issue 4, pp. e19114, 2011

Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, 2010

Neganova, Zhang, Atkinson, Lako: "Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells." in: Oncogene, Vol. 28, Issue 1, pp. 20-30, 2009

Maimets, Neganova, Armstrong, Lako: "Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells." in: Oncogene, Vol. 27, Issue 40, pp. 5277-87, 2008

Images

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Supplier Images
Western Blotting (WB) image for anti-Nanog Homeobox (NANOG) (Middle Region) antibody (ABIN2777175) WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...