anti-Pig (Porcine) HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
443
237
167
54
38
35
24
14
13
11
11
10
7
5
3
2
1
Host
Mouse
263
196
3
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
29
24
17
15
13
8
8
7
7
7
6
6
6
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
396
186
161
131
115
93
46
26
21
13
11
8
5
3
2
2
1
1
1
1
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Available images

Catalog No. ABIN4319335
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1084251 ABIN1107584 WB Mouse IgG2a Log in to see KS1 1
3.1084251 ABIN2957615 WB Rabbit N-Term, acLys12 Log in to see Polyclonal 0
3.1084251 ABIN2957616 IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.1084251 ABIN2957614 IC IF IHC WB Rabbit Log in to see Polyclonal 0
3.1084251 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3 0
1 ABIN324443 WB Rabbit IgG AA 126-175 Log in to see Polyclonal 0
1 ABIN203033 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN575653 WB Rabbit AA 21-70 Log in to see Polyclonal 0
1 ABIN615020 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1735506 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2621941 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2621942 IHC IHC (p) WB Rabbit AA 121-170 Log in to see Polyclonal 0
1 ABIN208594 WB Mouse IgG2a Log in to see 0
1 ABIN2467469 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4961219 FACS ICC IF WB DyLight 680 Mouse IgG2b Log in to see 1F3 0
1 ABIN4961226 FACS ICC IF WB DyLight 350 Mouse IgG2b Log in to see 1F3 0
1 ABIN4961227 FACS ICC IF WB DyLight 405 Mouse IgG2b Log in to see 1F3 0
1 ABIN4961211 FACS ICC IF WB Alexa Fluor 405 Mouse IgG2b Log in to see 1F3 0
1 ABIN4961222 FACS ICC IF WB DyLight 755 Mouse IgG2b Log in to see 1F3 0
1 ABIN4961213 FACS ICC IF WB Alexa Fluor 647 Mouse IgG2b Log in to see 1F3 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(443), (237), (167), (54), (38), (35), (24), (14), (13), (11), (11), (10), (7), (5), (3), (2), (1)
Host Mouse
(263), (196), (3), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(29), (24), (17), (15), (13), (8), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
(396), (186), (161), (131), (115), (93), (46), (26), (21), (13), (11), (8), (5), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Full length human HMGB1 [UniProt# P09429]
Clone 1F3
Isotype IgG2b
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Gene Symbol: HMGB1
Molecular Weight Theoretical MW: 25 kDa
Gene ID 3146
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Western Blot 1:1000-1:2000, Simple Western 1:500, Flow Cytometry 1 μg per million cells, Immunocytochemistry/Immunofluorescence 1:1000, CyTOF-readyThis HMGB1 (1F3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 25 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Simple Western (SimWes) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view sh...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Blot of crude HeLa cell extra...
Flow Cytometry (FACS) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometr...
Immunofluorescence (IF) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeL...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) - Analysis of of ...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN4319335) Western Blot: HMGB1/HMG-1 Antibody (1F3) - Analysis of lysates from different cell l...