|Antigen||Bone Morphogenetic Protein 7 (BMP7) Antibodies|
|Conjugate||This BMP7 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-BMP7 AntibodyTarget Details BMP7 Application Details Handling Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity Chromatography using an immobilized Human BMP7 matrix.|
|Immunogen||CHO cell derived recombinant highly pure (>98%) Human BMP-7.|
|Plasmids, Primers & others|
Target Details BMP7Product Details anti-BMP7 Antibody Application Details Handling Images back to top
|Alternative Name||BMP7 (BMP7 Antibody Abstract)|
|Background||Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1|
|Pathways||Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance|
Application DetailsProduct Details anti-BMP7 Antibody Target Details BMP7 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP7 (100 ng/mL), a concentration of 0.40-0.60 μg/mL of this BMP7 antibody is required. Sandwich ELISA: To detect Human BMP7 by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL is required. The antigen affinity purified BMP7 antibody, inconjunction with Biotinylated antibody anti-Human BMP7 antibody (ABIN181549 orABIN181550) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human BMP-7. Western Blot: To detect Humnan BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Hunan BMP-7 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BMP7 Antibody Target Details BMP7 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.|
|Expiry Date||6 months|