anti-Mouse (Murine) CD247 antibody for Cytometry by Time of Flight

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • CD3-ZETA
  • CD3H
  • CD3Q
  • CD3Z
  • T3Z
  • TCRZ
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • TCRzeta
  • CD247 molecule
  • CD247 antigen
  • CD3zeta-2
  • CD3zeta-1
  • Cd247 molecule
  • CD247
  • Cd247
  • cd3z
  • cd247
Reactivity
Mouse (Murine)
253
158
38
9
4
4
1
1
1
1
1
Host
Hamster
125
105
19
17
12
5
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
22
12
11
9
8
7
7
6
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunoprecipitation (IP)
199
162
116
50
46
41
22
18
5
4
4
3
2
1
1
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Catalog No. ABIN4900709
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900705 CyTOF FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
1 ABIN4291755 CyTOF FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968 0

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Mouse (Murine)
(253), (158), (38), (9), (4), (4), (1), (1), (1), (1), (1)
Host Hamster
(125), (105), (19), (17), (12), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(22), (12), (11), (9), (8), (7), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunoprecipitation (IP)
(199), (162), (116), (50), (46), (41), (22), (18), (5), (4), (4), (3), (2), (1), (1)
Pubmed 8 references available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN4900709) Images
Specificity Detects mouse CD3 epsilon . Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon-chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen H-2Kb-specific mouse cytotoxic T-lymphocyte
Clone 145-2C11
Isotype IgG
Plasmids, Primers & others

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 antibody (ABIN4900709) Images back to top
Antigen
Alternative Name Cd247 (CD247 Antibody Abstract)
Background Alternative Names: CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
Gene ID 916
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 antibody (ABIN4900709) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells
Immunoprecipitation
Cell depletion
T Cell Stimulation 0.1-0.6 μg/mL
CyTOF-reported
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 antibody (ABIN4900709) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD247 antibody (ABIN4900709)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Gupta, Marcel, Talwar, Garg, R, Perumalsamy, Sarin, Shivashankar: "Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration." in: PLoS ONE, Vol. 7, Issue 9, pp. e43718, 2012 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Jones, Stumhofer, Foster, Twohig, Hertzog, Topley, Williams, Hunter, Jenkins, Wang, Jones: "Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 409-19, 2011 Method employed by authors: Biochemical Assay (BCA) (Sample species: Mouse (Murine)).

Jones, McLoughlin, Hammond, Parker, Williams, Malhotra, Scheller, Williams, Rose-John, Topley, Jones: "Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 4, pp. 2130-9, 2010 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Sinclair, Finlay, Feijoo, Cornish, Gray, Ager, Okkenhaug, Hagenbeek, Spits, Cantrell: "Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking." in: Nature immunology, Vol. 9, Issue 5, pp. 513-21, 2008 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Li, Kashiwamura, Ueda, Sekiyama, Okamura: "Protection of CD8+ T cells from activation-induced cell death by IL-18." in: Journal of leukocyte biology, Vol. 82, Issue 1, pp. 142-51, 2007 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Bheeshmachar, Purushotaman, Sade, Gunasekharan, Rangarajan, Sarin: "Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5041-50, 2006 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Ogilvie, Abelson, Hau, Vlasova, Blackshear, Bohjanen: "Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 2, pp. 953-61, 2005 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Kipnis, Cardon, Avidan, Lewitus, Mordechay, Rolls, Shani, Schwartz: "Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 27, pp. 6133-43, 2004 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN4900709) back to top
Supplier Images
Functional Studies (Func) image for anti-CD247 Molecule (CD247) antibody (ABIN4900709) Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster An...