anti-Chicken CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Chicken, Turkey
496
422
103
69
67
42
41
34
26
23
15
7
3
3
3
2
1
1
1
Host
Mouse
526
203
92
77
40
30
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
113
95
74
38
22
21
20
20
18
18
17
13
13
13
13
12
12
12
8
8
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
794
210
185
160
143
142
133
130
88
77
26
21
13
9
8
6
6
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN371359
$ 357.50
Plus shipping costs $45.00

General

Antigen CD28 Antibodies
Reactivity Chicken, Turkey
(496), (422), (103), (69), (67), (42), (41), (34), (26), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(526), (203), (92), (77), (40), (30), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(113), (95), (74), (38), (22), (21), (20), (20), (18), (18), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(794), (210), (185), (160), (143), (142), (133), (130), (88), (77), (26), (21), (13), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN371359) Images
Specificity This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50 % amino acid sequence identity with mammalian CD28.
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Purification Purified
Clone AV7
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN371359) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 396249
UniProt P31043
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN371359) Images back to top
Application Notes Flow Cytometry. Immunoprecipitation. In vitro T-cell costimulation. (Ref.2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN371359) Images back to top
Concentration 0.5 mg/mL
Buffer 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD28 antibody (ABIN371359)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Young, Davison, Tregaskes, Rennie, Vainio: "Monomeric homologue of mammalian CD28 is expressed on chicken T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3848-51, 1994

Arstila, Vainio, Lassila: "Evolutionarily conserved function of CD28 in alpha beta T cell activation." in: Scandinavian journal of immunology, Vol. 40, Issue 3, pp. 368-71, 1994