|Reactivity||Chicken, Turkey Alternatives|
|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
|2 references available|
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Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN371359) Images
|Specificity||This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50 % amino acid sequence identity with mammalian CD28.|
|Characteristics||Synonyms: TP44, T-cell-specific surface glycoprotein CD28|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN371359) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN371359) Images back to top
Flow Cytometry. Immunoprecipitation. In vitro T-cell costimulation. (Ref.2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN371359) Images back to top
|Buffer||100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD28 antibody (ABIN371359)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Young, Davison, Tregaskes, Rennie, Vainio: "Monomeric homologue of mammalian CD28 is expressed on chicken T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3848-51, 1994
Arstila, Vainio, Lassila: "Evolutionarily conserved function of CD28 in alpha beta T cell activation." in: Scandinavian journal of immunology, Vol. 40, Issue 3, pp. 368-71, 1994