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|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
|4 references available|
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Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN5012855) Images
|Specificity||Detects human CD28 in direct ELISAs and Western blots. In direct ELISAs, approximately 20 % cross-reactivity with recombinant mouse CD28 is observed.|
|Purification||Immunogen affinity purified|
|Endotoxin Level||<0.10 EU per 1 μg of the antibody by the LAL method.|
|Immunogen||S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28|
|Plasmids, Primers & others|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN5012855) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||Alternative Names: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 Molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN5012855) Images back to top
Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Agonist Activity 0.3-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN5012855) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-CD28 antibody (ABIN5012855)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
|Product cited in:||
Ellinghaus, Cortini, Pinder, Le Friec, Kemper, Vyse: "Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus." in: European journal of immunology, Vol. 47, Issue 7, pp. 1200-1210, 2017 Method employed by authors: Functional Studies (Func) (Sample species: Human).
Boyette, Macedo, Hadi, Elinoff, Walters, Ramaswami, Chalasani, Taboas, Lakkis, Metes: "Phenotype, function, and differentiation potential of human monocyte subsets." in: PLoS ONE, Vol. 12, Issue 4, pp. e0176460, 2017 Method employed by authors: Functional Studies (Func) (Sample species: Human).
Bahlis, King, Kolonias, Carlson, Liu, Hussein, Terebelo, Byrne, Levine, Boise, Lee: "CD28-mediated regulation of multiple myeloma cell proliferation and survival." in: Blood, Vol. 109, Issue 11, pp. 5002-10, 2007 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).
Sakthivel, Shively, Kakoulidou, Pearce, Lefvert: "The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm." in: Journal of internal medicine, Vol. 261, Issue 4, pp. 399-407, 2007 Method employed by authors: ELISA (ELISA) (Sample species: Human).
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