anti-Human CD28 antibody for Immunocytochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Reactivity
Human, Non-Human Primate
426
353
86
43
35
25
25
16
12
6
6
2
2
2
2
1
1
1
Host
Mouse
425
182
96
37
31
31
7
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to PerCP
99
85
60
52
13
12
11
10
8
8
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
649
158
151
131
102
75
60
47
42
26
25
14
13
9
5
4
4
3
3
2
2
2
2
1
1
1
Options
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Catalog No. ABIN457388
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.28035 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
25.28035 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
25.28035 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
24.508373 ABIN5012855 Agon FACS ICC WB Goat IgG Log in to see Polyclonal 5
23.78035 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
20.78035 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
8.37283 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 1
7 ABIN6255852 ELISA ICC IF WB Rabbit IgG pTyr218 Log in to see Polyclonal 0
4 ABIN2618450 FACS Func ICC IHC IHC (fro) IHC (p) IP Golden Syrian Hamster IgG Log in to see 37-51 0
1 ABIN1959966 FACS Func ICC IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN1975063 ELISA ICC IF WB PE Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1970482 ELISA ICC IF WB FITC Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1972544 ELISA ICC IF WB HRP Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1961665 ELISA ICC IF WB Alkaline Phosphatase (AP) Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1964045 ELISA ICC IF WB APC Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1966662 ELISA ICC IF WB Biotin Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1967135 FACS Func ICC IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN3199704 FACS Func ICC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN3200891 FACS Func ICC IHC (fro) IP WB APC Mouse IgG1 Log in to see CD28-2 0
1 ABIN3200893 FACS Func ICC IHC (fro) IP WB PE Mouse IgG1 Log in to see CD28-2 0

General

Antigen CD28 Antibodies
Reactivity Human, Non-Human Primate
(426), (353), (86), (43), (35), (25), (25), (16), (12), (6), (6), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(425), (182), (96), (37), (31), (31), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to PerCP
(99), (85), (60), (52), (13), (12), (11), (10), (8), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(649), (158), (151), (131), (102), (75), (60), (47), (42), (26), (25), (14), (13), (9), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN457388) Images
Specificity The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
HLDA V., WS Code 5T CD28.05
Cross-Reactivity (Details) Human, Non-Human Primates
Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN457388) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN457388) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN457388) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD28 antibody (ABIN457388)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999

Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN457388) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 (CD28) antibody (PerCP) (ABIN457388) Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) p...
Flow Cytometry (FACS) image for anti-CD28 (CD28) antibody (PerCP) (ABIN457388) Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) P...