anti-Human CD28 antibody for Immunohistochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Epitope
Internal Region
17
16
15
12
12
11
11
7
7
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
568
393
96
49
44
43
43
36
16
7
6
2
2
2
2
1
1
1
Host
Goat
556
170
99
59
51
30
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
111
91
72
58
22
19
13
13
11
10
9
9
9
9
8
8
8
8
8
8
8
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
797
266
177
158
148
143
127
124
85
52
48
26
13
11
9
5
3
3
3
3
2
2
2
2
1
1
1
1
Options
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Catalog No. ABIN625822
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.072235 ABIN1383845 FACS IHC Mouse IgG2a Log in to see B-T3 6
20.072235 ABIN1383848 FACS IHC Mouse IgG2a Log in to see B-T3 1
12.972757 ABIN6260603 IHC WB Rabbit IgG Log in to see Polyclonal 0
6.9727573 ABIN2618450 FACS Func ICC IHC IHC (fro) IHC (p) IP Golden Syrian Hamster IgG Log in to see 37-51 0
5.5 ABIN610468 FACS IHC IHC (fro) Mouse IgG2a Log in to see 204-12 2
4 ABIN205423 FACS IHC IHC (fro) IP Mouse IgG1 Log in to see CD28-2 0
1 ABIN600765 FACS IHC IHC (fro) Mouse IgG2a Log in to see 204-12 0
1 ABIN203924 FACS IHC IHC (fro) IP Mouse IgG1, kappa Log in to see 0
1 ABIN600764 FACS IHC IHC (fro) Rat IgG2b Log in to see 0
1 ABIN1959966 FACS Func ICC IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN576560 FACS IHC IHC (fro) PE Rat IgG2b Log in to see 0
1 ABIN600763 FACS Func IHC IHC (fro) Rat IgG2b Log in to see 0
1 ABIN1967135 FACS Func ICC IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN2739194 FACS IF IHC IHC (p) Mouse IgG1, kappa Log in to see C28-77 0
1 ABIN2739192 FACS IF IHC IHC (p) Mouse IgG1, kappa Log in to see C28-75 0
1 ABIN2739193 ELISA FACS IF IHC IHC (fro) IP Mouse IgG1, kappa Log in to see CB28 0
1 ABIN217792 FACS Func IHC IHC (fro) Rat IgG2b Log in to see 0
1 ABIN211791 FACS IHC IHC (fro) Rat IgG2b Log in to see 0
1 ABIN6251878 FACS Func IHC IP Mouse IgG1, kappa Log in to see C28-75 0
1 ABIN6251879 FACS IF IHC WB Mouse IgG1, kappa Log in to see C28-77 0

General

Antigen CD28 Antibodies
Epitope Internal Region
(17), (16), (15), (12), (12), (11), (11), (7), (7), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(568), (393), (96), (49), (44), (43), (43), (36), (16), (7), (6), (2), (2), (2), (2), (1), (1), (1)
Host Goat
(556), (170), (99), (59), (51), (30), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(111), (91), (72), (58), (22), (19), (13), (13), (11), (10), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(797), (266), (177), (158), (148), (143), (127), (124), (85), (52), (48), (26), (13), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Purpose CD28
Blocking Peptide Blocking peptide for this product available: ABIN2815357
Sequence SQQLQVYSKT GFNCD
Specificity The immunizing peptide corresponds to a part of the extracellular domain.
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen SQQLQVYSKTGFNCD
Plasmids, Primers & others

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28, CD28 Molecule, MGC138290, Tp44, CD28 antigen, CD28 antigen (Tp44), T-cell-specific surface glycoprotein
Gene ID 940
NCBI Accession NP_006130
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes

DS WB Results: Approx 48 kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD28 (CD28) (Internal Region) antibody (ABIN625822) Antibody (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen ...
 image for anti-CD28 (CD28) (Internal Region) antibody (ABIN625822) Antibody Negative Control showing staining of paraffin embedded Human Lymph Node, wit...