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|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD28 antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS)
|1 reference available|
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Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN120437) Images
|Specificity||Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).|
|Purification||Protein G Chromatography|
|Immunogen||Rat CD28 transfected A20J cells|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN120437) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN120437) Images back to top
Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN120437) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.|
|Expiry Date||6 months|
References for anti-CD28 antibody (ABIN120437)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Tacke, Clark, Dallman, Hünig et al.: "Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 10, pp. 5121-7, 1995
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN120437) back to top