We are preparing the requested document. Please wait, this may take a while...!
|Antigen||CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies|
|Epitope||AA 1-105 Alternatives|
|Conjugate||This CD3D antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CD3D AntibodyTarget Details CD3D Application Details Handling Images
|Characteristics||Produced in rabbits immunized with purified, recombinant Human CD3D (rh CD3D, ABIN2003686 NP_000723.1, Met 1-Ala 105,. CD3D specific IgG was purified by human CD3D affinity chromatography.|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human CD3D protein (ABIN2003686)|
Target Details CD3DProduct Details anti-CD3D Antibody Application Details Handling Images back to top
|Alternative Name||CD3D (CD3D Antibody Abstract)|
|Background||T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor- positive T cells is also impaired.|
|Pathways||TCR Signaling, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD3D Antibody Target Details CD3D Handling Images back to top
WB: 5-10 μg/mL
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD3D. The detection limit for Human CD3D is 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3D Antibody Target Details CD3D Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
ImagesProduct Details anti-CD3D Antibody Target Details CD3D Application Details Handling back to top