anti-Human CD8 antibody for Immunohistochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Reactivity
Human, Mouse (Murine)
600
455
125
91
47
24
15
15
15
13
12
9
6
4
2
2
2
2
2
2
2
1
1
Host
Rat
694
404
267
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
147
142
85
84
32
28
27
26
21
19
18
18
17
17
17
17
17
17
15
14
14
12
12
11
11
10
8
7
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
1107
442
293
251
250
214
207
153
147
29
20
20
18
13
13
11
10
6
6
5
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN268292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13 ABIN5996950 IF/ICC IHC WB Rabbit IgG Log in to see 0
12.900204 ABIN3030431 FACS IHC ELISA WB Rabbit Ig Fraction AA 150-180 Log in to see Polyclonal 0
12.900204 ABIN3028555 IHC ELISA WB Rabbit Ig Fraction AA 59-88 Log in to see Polyclonal 0
12.900204 ABIN6292505 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.900204 ABIN2997766 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.900204 ABIN2997767 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
12.461315 ABIN6135594 IF IHC WB Rabbit Log in to see 0
12.357094 ABIN4296028 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 1
10 ABIN1491225 IHC IHC (p) WB Mouse IgG1 AA 22-182 Log in to see 3H6 0
9.900204 ABIN6383871 IHC Mouse IgG1 Log in to see rC8-468 0
9.900204 ABIN462597 IHC IHC (fro) IHC (p) Rabbit C-Term Log in to see Polyclonal 0
9.900204 ABIN213677 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
9.900204 ABIN6383870 IHC Mouse IgG1 Log in to see rC8-468 0
7.8570943 ABIN4295973 IHC IHC (p) Mouse IgG1 Log in to see CL1529 0
7 ABIN1077936 ICC IHC WB Rabbit IgG AA 22-198 Log in to see Polyclonal 0
7 ABIN2896218 IF/ICC IHC IP WB Rabbit AA 22-198 Log in to see Polyclonal 0
6.900204 ABIN2469570 IHC Rabbit C-Term Log in to see Polyclonal 0
6.900204 ABIN5574862 ELISA IHC WB Rabbit Log in to see Polyclonal 0
4 ABIN475572 FACS IHC IHC (fro) Mouse IgG1, kappa Log in to see RPA-T8 0
4 ABIN2659757 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(600), (455), (125), (91), (47), (24), (15), (15), (15), (13), (12), (9), (6), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(694), (404), (267), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(147), (142), (85), (84), (32), (28), (27), (26), (21), (19), (18), (18), (17), (17), (17), (17), (17), (17), (15), (14), (14), (12), (12), (11), (11), (10), (8), (7), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1107), (442), (293), (251), (250), (214), (207), (153), (147), (29), (20), (20), (18), (13), (13), (11), (10), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN268292) Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a
Plasmids, Primers & others

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN268292) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN268292) Images back to top
Application Notes Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN268292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 antibody (ABIN268292)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Priego, Zhu, Monteiro, Mulders, Wasilewski, Bindeman, Doglio, Martínez, Martínez-Saez, Cajal, Megías, Hernández-Encinas, Blanco-Aparicio, Martínez, Zarzuela, Muñoz, Fustero-Torre, Piñeiro-Yáñez et al.: "STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis. ..." in: Nature medicine, Vol. 24, Issue 7, pp. 1024-1035, 2018

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Karlsson, Johansen, Kahu, Macpherson, Brandtzaeg: "Hypersensitivity and oral tolerance in the absence of a secretory immune system." in: Allergy, Vol. 65, Issue 5, pp. 561-70, 2010

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009

Himoudi, Nabarro, Yan, Gilmour, Thrasher, Anderson: "Development of anti-PAX3 immune responses; a target for cancer immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 9, pp. 1381-95, 2007

Auray, Lacroix-Lamandé, Mancassola, Dimier-Poisson, Laurent: "Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection." in: Microbes and infection, Vol. 9, Issue 5, pp. 574-82, 2007

Sroga, Jones, Kigerl, McGaughy, Popovich: "Rats and mice exhibit distinct inflammatory reactions after spinal cord injury." in: The Journal of comparative neurology, Vol. 462, Issue 2, pp. 223-40, 2003

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN268292) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) anti-CD8a Molecule (CD8A) antibody
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Staining of Mouse...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Published ...
Immunofluorescence (IF) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) [ABIN268292] -...
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) - Staining of a mouse lymph nod...