anti-Mouse (Murine) CD8 antibody for ELISA

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Reactivity
Human, Mouse (Murine)
600
505
128
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
767
465
199
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to DyLight 594
151
144
92
85
46
36
36
33
27
24
23
23
23
23
23
20
19
19
18
15
15
13
11
11
10
9
7
7
6
5
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1162
450
366
256
243
239
201
188
174
35
35
20
16
13
13
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN5630736
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN610245 FACS ELISA Biotin Rat IgG2a, kappa Log in to see 53-6-72 2
1 ABIN610246 FACS ELISA FITC Rat IgG2a, kappa Log in to see 53-6-72 2
1 ABIN2000853 ELISA Rabbit IgG AA 1-196, Extracellular Domain Log in to see Polyclonal 0
1 ABIN2000851 ELISA Rabbit IgG AA 1-196, Extracellular Domain Log in to see Polyclonal 0
1 ABIN610247 FACS ELISA PE Rat IgG2a, kappa Log in to see 53-6-72 0
1 ABIN610244 FACS ELISA Rat IgG2a, kappa Log in to see 53-6-72 0
1 ABIN2000845 ELISA Rabbit IgG AA 1-196, Extracellular Domain Log in to see 012 0
1 ABIN2739735 FACS ELISA APC Rat IgG2a, kappa Log in to see 53-6-72 0
1 ABIN2739737 FACS ELISA Cy7,PE Rat IgG2a, kappa Log in to see 53-6-72 0
1 ABIN2739736 FACS ELISA DyLight 350 Rat IgG2a, kappa Log in to see 53-6-72 0
1 ABIN5693154 ELISA FACS ICC IHC WB Rabbit AA 28-196 Log in to see Polyclonal 0
1 ABIN2593828 ELISA WB Rabbit IgG AA 27-247 Log in to see Polyclonal 0
1 ABIN2896220 ELISA IF/ICC IHC WB Rabbit AA 27-247 Log in to see Polyclonal 0

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(600), (505), (128), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(767), (465), (199), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to DyLight 594
(151), (144), (92), (85), (46), (36), (36), (33), (27), (24), (23), (23), (23), (23), (23), (20), (19), (19), (18), (15), (15), (13), (11), (11), (10), (9), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1162), (450), (366), (256), (243), (239), (201), (188), (174), (35), (35), (20), (16), (13), (13), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 50 mM Sodium Borate
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (DyLight 594) (ABIN5630736) Flow Cytometry: CD8 alpha Antibody (YTS105.18) - Staining of mouse spleen cells.
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-CD8a Molecule (CD8A) antibody (DyLight 594) (ABIN5630736) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) - Staining o...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (DyLight 594) (ABIN5630736) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) - Staining of (A) Infil...
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (DyLight 594) (ABIN5630736) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) - Staining of Mouse lymph node...