anti-Mouse (Murine) CD8 antibody for Immunoprecipitation

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Reactivity
Mouse (Murine)
601
505
128
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
767
465
199
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
151
144
92
85
46
36
36
33
27
24
23
23
23
23
23
20
19
19
18
15
15
13
11
11
10
9
7
7
6
5
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
1162
450
366
257
242
240
202
189
175
35
35
20
16
13
12
10
9
7
6
6
5
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2689432
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2749035 FACS Func ICC IHC (f) IHC (p) IP Rat IgG2a Log in to see 53-6-7 8
1 ABIN2749187 Func IHC (f) ICC FACS IP IHC (p) Rat IgG2a Log in to see 53-6-7 8
1 ABIN1177008 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53-6-7 25
1 ABIN4295972 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7 9
1 ABIN4900731 CyTOF FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
1 ABIN4900730 CyTOF FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
1 ABIN2660735 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10
1 ABIN2657489 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
1 ABIN2658875 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 10
1 ABIN457303 FACS Func ICC IHC (f) IHC (p) IP APC Rat IgG2a Log in to see 53-6-7 7
1 ABIN2660736 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 12
1 ABIN349708 FACS Func ICC IHC (f) IHC (p) IP FITC Rat IgG2a Log in to see 53-6-7 9
1 ABIN2658876 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 11
1 ABIN349711 FACS Func ICC IHC (f) IHC (p) IP PE Rat IgG2a Log in to see 53-6-7 8
1 ABIN2657490 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
1 ABIN135118 FACS IP IHC Rat IgG2a Log in to see 53-6-7 6
1 ABIN135121 FACS IP IHC Biotin Rat IgG2a Log in to see 53-6-7 5
1 ABIN153109 FACS IHC (fro) IP IHC Rat IgG1 kappa Log in to see IBL-3-25 0
1 ABIN593713 FACS IHC (fro) IP Cy5 Rat IgG2a Log in to see 53-6-7 0
1 ABIN593711 FACS IHC (fro) IP Cy7,PE Rat IgG2a Log in to see 53-6-7 0

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(601), (505), (128), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(767), (465), (199), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(151), (144), (92), (85), (46), (36), (36), (33), (27), (24), (23), (23), (23), (23), (23), (20), (19), (19), (18), (15), (15), (13), (11), (11), (10), (9), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
(1162), (450), (366), (257), (242), (240), (202), (189), (175), (35), (35), (20), (16), (13), (12), (10), (9), (7), (6), (6), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 24 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN2689432) Images
Brand BD Pharmingen™
Characteristics The 53-6.7 antibody has been reported to react with the 38 kDa α and 34 kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 α and α' chains arise from alternatively spliced messengers of a single CD8a gene. The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated α' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD8a - Purified - Clone 53-6.7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse thymus / spleen
Clone 53
Isotype IgG2a, kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN2689432) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background Synonyms: Ly-2, Lyt-2
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN2689432) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN2689432) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD8 antibody (ABIN2689432)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Leishman, Naidenko, Attinger, Koning, Lena, Xiong, Chang, Reinherz, Kronenberg, Cheroutre: "T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL." in: Science (New York, N.Y.), Vol. 294, Issue 5548, pp. 1936-9, 2001

Traver, Akashi, Manz, Merad, Miyamoto, Engleman, Weissman: "Development of CD8alpha-positive dendritic cells from a common myeloid progenitor." in: Science (New York, N.Y.), Vol. 290, Issue 5499, pp. 2152-4, 2000

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Negm, Mansour, Saad, Abdel Halim: "Structural characterization of an Lyt-2/3 homolog expressed on Bufo regularis lymphocytes." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 113, Issue 1, pp. 79-87, 1997

Alexander-Miller, Leggatt, Sarin, Berzofsky: "Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 485-92, 1996

Anel, ORourke, Kleinfeld, Mescher: "T cell receptor and CD8-dependent tyrosine phosphorylation events in cytotoxic T lymphocytes: activation of p56lck by CD8 binding to class I protein." in: European journal of immunology, Vol. 26, Issue 10, pp. 2310-9, 1996

Fujiura, Kawaguchi, Kondo, Obana, Yamamoto, Nanno, Ishikawa: "Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, 1996

Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, 1996

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995

Nakayama, Nakayama, Negishi, Kuida, Louie, Kanagawa, Nakauchi, Loh: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, 1994

Wang, Klein: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, 1994

ORourke, Mescher: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, 1993

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992

Murosaki, Yoshikai, Ishida, Nakamura, Matsuzaki, Takimoto, Yuuki, Nomoto: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, 1992

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

MacDonald, Schreyer, Howe, Bron: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, 1990

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Zamoyska, Derham, Gorman, von Hoegen, Bolen, Veillette, Parnes: "Inability of CD8 alpha' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo." in: Nature, Vol. 342, Issue 6247, pp. 278-81, 1989

Zamoyska, Vollmer, Sizer, Liaw, Parnes: "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA." in: Cell, Vol. 43, Issue 1, pp. 153-63, 1986