anti-Rat (Rattus) CD86 antibody for Flow Cytometry

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
419
179
10
6
3
2
2
1
1
Host
Mouse
385
307
110
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
97
94
55
47
20
14
13
11
10
9
8
7
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
610
258
178
171
132
104
90
68
55
53
43
13
13
12
11
10
8
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN3025928
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.582786 ABIN3024504 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CDLA86 0
14.082786 ABIN2472686 IHC (fro) FACS IP Mouse IgG1 Log in to see 24F 4
13.25679 ABIN2137061 FACS FITC Mouse IgG1 kappa Log in to see 24F 0
12.582786 ABIN3026659 FACS IF IHC (p) Mouse IgG1, kappa Log in to see BU63 0
12.558315 ABIN5951757 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SJ20-00 0
10 ABIN6185458 FACS IF IHC (f) WB APC Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185465 FACS IF IHC (f) WB PerCP Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185466 FACS IF IHC (f) WB PE Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185469 FACS IF IHC (f) WB Mouse IgG1, kappa Log in to see C86-1146 0
9.582786 ABIN2657855 FACS ELISA Alexa Fluor 647 Mouse IgG1 kappa Log in to see 24F 7
9.582786 ABIN2663616 BR CoSt FACS IP IHC PE Mouse IgG1 kappa Log in to see 24F 6
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Log in to see BU63 0
5.5 ABIN2689425 FACS Biotin Mouse IgG1, kappa Log in to see 24F 3
5.5 ABIN2689421 IHC (fro) FACS IP Mouse IgG1, kappa Log in to see 24F 3
5.5 ABIN2472696 Func IHC (fro) FACS IP Mouse IgG1 Log in to see 24F 4
5.5 ABIN2472688 FACS Alexa Fluor 488 Mouse IgG1 Log in to see 24F 4
5.5 ABIN2472690 FACS Alexa Fluor 647 Mouse IgG1 Log in to see 24F 4

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(456), (419), (179), (10), (6), (3), (2), (2), (1), (1)
Host Mouse
(385), (307), (110), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(97), (94), (55), (47), (20), (14), (13), (11), (10), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(610), (258), (178), (171), (132), (104), (90), (68), (55), (53), (43), (13), (13), (12), (11), (10), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
Clone C86-1146
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background Recognizes a protein of 70  kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling Images back to top
Application Notes Optimal dilution of the CD86 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN3025928) Flow cytometry testing of human PBMC using CD86 antibody (C86/1146).
Immunohistochemistry (IHC) image for anti-CD86 Molecule (CD86) antibody (ABIN3025928) Formalin-fixed, paraffin-embedded human melanoma stained with CD86 antibody (C86/1146).
Western Blotting (WB) image for anti-CD86 Molecule (CD86) antibody (ABIN3025928) Western blot analysis of Daudi cell lysate using CD86 antibody (C86/1146).