anti-Rat (Rattus) CD86 antibody for Flow Cytometry

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Human, Mouse (Murine), Rat (Rattus)
476
436
178
10
6
4
2
2
1
1
Host
Mouse
395
326
114
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
98
98
57
52
21
17
16
14
10
10
9
9
8
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
640
266
179
179
134
106
90
68
55
54
43
14
13
12
11
10
8
4
2
2
2
1
1
1
1
Options
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Available images

Catalog No. ABIN3024504
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.6854515 ABIN3025928 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see C86-1146 0
14.1854515 ABIN2472686 IHC (fro) FACS IP Mouse IgG1 Log in to see 24F 4
12.6854515 ABIN3026659 FACS IF IHC (p) Mouse IgG1, kappa Log in to see BU63 0
12.6854515 ABIN3024505 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CDLA86 0
12.6854515 ABIN3025929 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see C86-1146 0
12.026281 ABIN2137061 FACS FITC Mouse IgG1 kappa Log in to see 24F 0
11.145817 ABIN5951757 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SJ20-00 0
10 ABIN6185458 FACS IF IHC (f) WB APC Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185465 FACS IF IHC (f) WB PerCP Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185466 FACS IF IHC (f) WB PE Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185469 FACS IF IHC (f) WB Mouse IgG1, kappa Log in to see C86-1146 0
9.6854515 ABIN2657855 FACS ELISA Alexa Fluor 647 Mouse IgG1 kappa Log in to see 24F 7
9.6854515 ABIN2663616 BR CoSt FACS IP IHC PE Mouse IgG1 kappa Log in to see 24F 6
9.6854515 ABIN3026658 FACS IF Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Log in to see BU63 0
5.5 ABIN2689425 FACS Biotin Mouse IgG1, kappa Log in to see 24F 3
5.5 ABIN2689421 IHC (fro) FACS IP Mouse IgG1, kappa Log in to see 24F 3

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(476), (436), (178), (10), (6), (4), (2), (2), (1), (1)
Host Mouse
(395), (326), (114), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(98), (98), (57), (52), (21), (17), (16), (14), (10), (10), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(640), (266), (179), (179), (134), (106), (90), (68), (55), (54), (43), (14), (13), (12), (11), (10), (8), (4), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
Clone CDLA86
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling Images back to top
Application Notes Optimal dilution of the CD86 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN3024504) Flow cytometry testing of human PBMC using CD86 antibody (clone CDLA86).
Immunohistochemistry (IHC) image for anti-CD86 Molecule (CD86) antibody (ABIN3024504) IHC testing of FFPE human melanoma with CD86 antibody (clone CDLA86).
Western Blotting (WB) image for anti-CD86 Molecule (CD86) antibody (ABIN3024504) Western blot testing of Daudi cell lysate using CD86 antibody (clone CDLA86). Observe...