anti-Human Cytoplasmic Protein NCK1 antibody for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Epitope
N-Term
24
20
10
9
7
5
4
3
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
153
35
26
18
7
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
86
66
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
147
96
69
27
14
13
8
6
5
2
1
1
1
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Catalog No. ABIN2789859
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0788064 ABIN393431 ELISA WB Mouse IgG1 kappa AA 185-294 Log in to see 1A1 5
3.0788064 ABIN657648 IHC (p) WB Rabbit Ig Fraction AA 63-92, N-Term Log in to see Polyclonal 3
3.0788064 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
3.0788064 ABIN652772 FACS WB Rabbit Ig Fraction AA 3-29, N-Term Log in to see Polyclonal 1
3.0788064 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
3.0788064 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN4338263 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
3.0788064 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
3.0788064 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
3.0788064 ABIN453756 EIA WB Rabbit N-Term Log in to see Polyclonal 1
3.0788064 ABIN4338266 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see 6B3 0
3.0788064 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1 0
3.0788064 ABIN1327536 IF WB Mouse AA 1-377, full length Log in to see Polyclonal 0
3.0788064 ABIN1873839 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN518212 IF WB Mouse AA 1-377, full length Log in to see Polyclonal 0
3.0788064 ABIN5970018 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN2989000 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN5963264 IF WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal 0
3.0788064 ABIN4338265 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope N-Term
(24), (20), (10), (9), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(153), (35), (26), (18), (7), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(86), (66), (1)
Clonality
Polyclonal
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Western Blotting (WB)
(147), (96), (69), (27), (14), (13), (8), (6), (5), (2), (1), (1), (1)
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN980525
Sequence PSNYVERKNS ARKASIVKNL KDTLGIGKVK RKPSVPDSAS PADDSFVDPG
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against NCK1. It was validated on Western Blot.
Purification Affinity Purified
Plasmids, Primers & others

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Alias Symbols: MGC12668, NCK, NCKalpha, nck-1
Protein Interaction Partner: UBC, CBL, SYNPO, WAS, Dok1, HSPA5, Mbp, SHANK3, KIAA1522, Caskin1, LPHN2, ADAM15, TUBA1A, ERBB3, EGFR, PHC2, FGFR1, MAP4K1, BCAR1, nef, ABI1, PAK1, DNM1, ABL1, TNK2, FASLG, UBASH3B, SYK, PIK3R1, LCP2, INPP5D, GRB2, KHDRBS1, UBA52, RASA1, NCKAP5, TNIK, BRK
Protein Size: 377
Molecular Weight 42 kDa
Gene ID 4690
NCBI Accession NM_006153, NP_006144
UniProt P16333
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 377 AA

Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (N-Term) antibody (ABIN2789859) WB Suggested Anti-NCK1 Antibody Titration: 1.0 ug/ml Positive Control: NCI-H226 Whole...