anti-Human Replication Factor C (Activator 1) 3, 38kDa antibody for Enzyme Immunoassay

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Epitope
Middle Region
7
6
5
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
60
27
25
9
8
5
4
3
3
3
3
2
1
1
1
Host
Rabbit
49
10
1
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
58
22
14
10
9
7
4
3
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Catalog No. ABIN358042
$ 390.50
Plus shipping costs $45.00

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Epitope Middle Region
(7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(60), (27), (25), (9), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(49), (10), (1)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(58), (22), (14), (10), (9), (7), (4), (3)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody detects RFC3 (Center).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human RFC3.
Isotype Ig Fraction

Target Details

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Antigen
Alternative Name RFC3 / RFC38 (RFC3 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.Synonyms: Activator 1 38 kDa subunit, Activator 1 subunit 3, RF-C 38 kDa subunit, Replication factor C 38 kDa subunit, Replication factor C subunit 3
Molecular Weight 40556 Da
Gene ID 5983, 9606
UniProt P40938
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References

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Background publications

Rauen, Burtelow, Dufault, Karnitz: "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9." in: The Journal of biological chemistry, Vol. 275, Issue 38, pp. 29767-71, 2000

Images

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Supplier Images
 image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (Middle Region) antibody (ABIN358042) anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (Middle Region) antibody