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General |
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Antigen | Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies |
Epitope | Middle Region Alternatives |
Reactivity | Human Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Application |
Enzyme Immunoassay (EIA), Western Blotting (WB)
Alternatives
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1 reference available |
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Specificity | This antibody detects RFC3 (Center). |
Purification | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human RFC3. |
Isotype | Ig Fraction |
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Antigen | |
Alternative Name | RFC3 / RFC38 (RFC3 Antibody Abstract) |
Background | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.Synonyms: Activator 1 38 kDa subunit, Activator 1 subunit 3, RF-C 38 kDa subunit, Replication factor C 38 kDa subunit, Replication factor C subunit 3 |
Molecular Weight | 40556 Da |
Gene ID | 5983, 9606 |
UniProt | P40938 |
Pathways | Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA |
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Application Notes |
ELISA: 1/1,000. Western Blot: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
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Format | Liquid |
Concentration | 0.25 mg/mL |
Buffer | PBS with 0.09 % (W/V) Sodium Azide as preservative. |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. |
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Background publications |
Rauen, Burtelow, Dufault, Karnitz: "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9." in: The Journal of biological chemistry, Vol. 275, Issue 38, pp. 29767-71, 2000 |
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