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|Antigen||Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects endogenous levels of RFC3 protein. (region surrounding Leu227)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
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|Alternative Name||RFC3 / RFC38 (RFC3 Antibody Abstract)|
|Background||The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: Activator 1 38 kDa subunit, Activator 1 subunit 3, RF-C 38 kDa subunit, Replication factor C 38 kDa subunit, Replication factor C subunit 3|
|Molecular Weight||approx. 41 kDa|
|Pathways||Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA|
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
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