anti-Dog (Canine) GNAS antibody for Immunoprecipitation

Recommended GNAS Antibody (supplied by: Log in to see )

Antigen
GNAS Complex Locus (GNAS) Antibodies
  • AHO
  • C20orf45
  • GNAS1
  • GPSA
  • GSA
  • GSP
  • NESP
  • PHP1A
  • PHP1B
  • PHP1C
  • POH
  • GNAS
  • PORGSA1
  • G-alpha-S
  • 5530400H20Rik
  • A930027G11Rik
  • C130027O20Rik
  • Galphas
  • Gnas1
  • Gnasxl
  • Gsa
  • Nesp
  • Nespl
  • Oed-Sml
  • Oedsml
  • P1
  • P2
  • P3
  • G-alpha-s
  • gnas-a
  • Gnpas
  • Nesp55
  • gnal
  • Gnas
  • GNAS complex locus
  • guanine nucleotide-binding protein G(s) subunit alpha
  • GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus
  • GNAS complex locus S homeolog
  • neuroendocrine secretory protein 55-like
  • GNAS
  • LOC694289
  • LOC469986
  • Gnas
  • gnas.S
  • gnas
  • LOC100346323
  • LOC100442753
  • LOC100732436
Epitope
AA 1028-1037
27
14
11
11
11
9
8
8
7
6
5
5
4
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Hamster, Horse (Equine), Human, Insect, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
168
59
42
13
10
8
7
6
6
6
3
3
3
2
1
Host
Rabbit
142
16
15
Clonality
Polyclonal
Conjugate
This GNAS antibody is un-conjugated
9
9
9
7
7
7
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
161
85
49
26
20
18
10
4
4
3
2
1
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Catalog No. ABIN228262
$ 837.83
Plus shipping costs $45.00

General

Antigen GNAS Complex Locus (GNAS) Antibodies
Epitope AA 1028-1037
(27), (14), (11), (11), (11), (9), (8), (8), (7), (6), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Hamster, Horse (Equine), Human, Insect, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(168), (59), (42), (13), (10), (8), (7), (6), (6), (6), (3), (3), (3), (2), (1)
Host Rabbit
(142), (16), (15)
Clonality
Polyclonal
Conjugate This GNAS antibody is un-conjugated
(9), (9), (9), (7), (7), (7)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(161), (85), (49), (26), (20), (18), (10), (4), (4), (3), (2), (1)
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Product Details anti-GNAS Antibody

Target Details GNAS Application Details Handling Images
Specificity Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
Purification Protein A purified
Immunogen Synthetic peptide corresponding to the C-terminal region of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotype IgG
Plasmids, Primers & others

Target Details GNAS

Product Details anti-GNAS Antibody Application Details Handling Images back to top
Antigen
Alternative Name GNAS (GNAS Antibody Abstract)
Background Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI
Gene ID 2778
Pathways Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis

Application Details

Product Details anti-GNAS Antibody Target Details GNAS Handling Images back to top
Application Notes Approved: ICC (1:100), IP, WB (1:500 - 1:1000)

Usage: Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20 μg of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2 μL immunoprecipitates Gs alpha(45kD) from 500 μg of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100 % ice-cold acetone.Optimal dilutions to be determined by researcher.
Comment

Target Species of Antibody: Bovine

Restrictions For Research Use only

Handling

Product Details anti-GNAS Antibody Target Details GNAS Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.