anti-Human TLR4 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Epitope
AA 780-830
59
30
28
25
16
15
15
15
14
13
11
9
8
8
7
6
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
555
274
173
94
72
72
64
26
12
9
4
2
1
1
Host
Rabbit
339
273
47
7
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
47
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
393
340
186
136
130
124
98
92
34
31
26
26
25
21
20
16
14
8
7
4
4
3
2
1
1
1
Options
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Catalog No. ABIN736491
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN4914683 IF (p) IHC (p) Rabbit IgG AA 155-185 Log in to see Polyclonal 0
5.5 ABIN917904 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 780-830 Log in to see Polyclonal 1
1 ABIN917902 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN917903 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN917905 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN736495 IF (cc) IF (p) Cy5 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN736496 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN736497 IF (cc) IF (p) Cy7 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN736494 IF (cc) IF (p) Cy3 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN736498 IF (cc) IF (p) FITC Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN2809068 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 780-830 Log in to see Polyclonal 0
1 ABIN4914694 IF (p) FITC Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914687 IF (p) Alexa Fluor 555 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914685 IF (p) Alexa Fluor 350 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914688 IF (p) Alexa Fluor 594 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914689 IF (p) Alexa Fluor 647 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914690 IF (p) Cy3 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914692 IF (p) Cy5.5 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914686 IF (p) Alexa Fluor 488 Rabbit IgG AA 155-185 Log in to see Polyclonal 0
1 ABIN4914693 IF (p) Cy7 Rabbit IgG AA 155-185 Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Epitope AA 780-830
(59), (30), (28), (25), (16), (15), (15), (15), (14), (13), (11), (9), (8), (8), (7), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(555), (274), (173), (94), (72), (72), (64), (26), (12), (9), (4), (2), (1), (1)
Host Rabbit
(339), (273), (47), (7)
Clonality
Polyclonal
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (47), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(393), (340), (186), (136), (130), (124), (98), (92), (34), (31), (26), (26), (25), (21), (20), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN736491) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat TLR4
Isotype IgG
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN736491) Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Subcellular location: Cytoplasm

Synonyms: Lps, Ly87, Ran/M1, Rasl2-8, Toll-like receptor 4, CD284, Tlr4

Gene ID 21898
UniProt Q9QUK6
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN736491) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN736491) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-TLR4 antibody (ABIN736491)

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
Product cited in:

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, 2018 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Harasymowicz, Clement, Azfer, Burnett, Salter, Simpson: "Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 69, Issue 7, pp. 1396-1406, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: adipose

Xu, Zheng, Liu, Li, Yang, Shang: "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 6, pp. 2091-2104, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: brain

Arfian, Muflikhah, Soeyono, Sari, Tranggono, Anggorowati, Romi: "Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis." in: The Kobe journal of medical sciences, Vol. 62, Issue 2, pp. E38-44, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: kidney

Kawakita, Fujimoto, Liu, Nakano, Nakatsuka, Suzuki: "Effects of Toll-Like Receptor 4 Antagonists Against Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Mice." in: Molecular neurobiology, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: brain

Wang, Shi, Lv, Xu, Li, Ge, Xiao, Geng, Liu: "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-?B Activation in Microglial Cells." in: Inflammation, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Namisaki, Noguchi, Moriya, Kitade, Aihara, Douhara, Nishimura, Takeda, Okura, Kawaratani, Takaya, Seki, Yoshiji: "Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis." in: Journal of gastroenterology, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: Intestine

Yang, Wang, Zhang, Yang, Lu, Zhang, Li, Zhang, Li: "Astragaloside IV attenuates inflammatory cytokines by inhibiting TLR4/NF-?B signaling pathway in isoproterenol-induced myocardial hypertrophy." in: Journal of ethnopharmacology, Vol. 150, Issue 3, pp. 1062-70, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: left ventricle and neonatal cardiomyocytes

Chen, Yu, Fan, Bian, Ma, Lu, Jin: "Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-?B) and induces cytokine production via Toll-like receptors." in: Carbohydrate polymers, Vol. 105, pp. 113-20, 2014 (Sample species: Rat (Rattus)).

Xing, Zheng, Zhang, Wang, Xing: "Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis." in: The Journal of international medical research, Vol. 42, Issue 4, pp. 958-965, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: Kidney

Takanashi, Tomosada, Villena, Murata, Takahashi, Chiba, Tohno, Shimazu, Aso, Suda, Ikegami, Itoh, Kawai, Saito, Alvarez, Kitazawa: "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." in: BMC microbiology, Vol. 13, pp. 54, 2013

Tang, Zhou, Wang, Zhang, Wang, Li, Huang: "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." in: Archives of oral biology, Vol. 56, Issue 10, pp. 1064-72, 2011 (Sample species: Human).

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN736491) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD2...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Po...
SDS-PAGE (SDS) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Mouse heart lysates probed with Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (AB...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Mouse macrophages(RAW264.7) labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, ...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Mouse splenocytes probed with Rabbit Anti-TLR4 Polyclonal Antibody .
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Image submitted by One World Lab validation program. HL60 and MCF-7 cells were staine...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Mouse macrophages(RAW264.7)labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, U...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) (AA 780-830) antibody (ABIN736491) Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD2...