anti-Rat (Rattus) TLR4 antibody for ELISA

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Reactivity
Human, Mouse (Murine), Rat (Rattus)
555
274
173
94
72
72
64
27
12
9
4
2
1
1
Host
Rabbit
339
273
47
7
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
47
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
393
340
185
137
129
123
97
92
34
31
27
26
26
21
20
16
14
8
7
4
4
3
2
1
1
1
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Catalog No. ABIN6269449
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN2427615 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN2422733 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
7 ABIN2429329 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
4 ABIN2421029 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN5701601 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5701602 ELISA IHC WB Mouse IgG2b Log in to see 0
1 ABIN6044116 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6044731 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1173524 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1093596 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2903163 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6102873 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN5907847 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(555), (274), (173), (94), (72), (72), (64), (27), (12), (9), (4), (2), (1), (1)
Host Rabbit
(339), (273), (47), (7)
Clonality
Polyclonal
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (47), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(393), (340), (185), (137), (129), (123), (97), (92), (34), (31), (27), (26), (26), (21), (20), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN6269449) Images
Specificity TLR4 antibody detects endogenous levels of total TLR4
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human TLR4
Isotype IgG
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN6269449) Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background

Description: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634, PubMed:27022195). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed:16622205).

Gene: TLR4

Molecular Weight 100kDa
Gene ID 7099
UniProt O00206
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN6269449) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN6269449) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-TLR4 antibody (ABIN6269449)

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
Product cited in:

Zhang, Fei, Tao, Li, Shen, Wang, Liu, Xu: "Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3848595, 2018

Huang, Chen, Zhang, Li, Chen, Yang, Liang, Zhu, Xu: "Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation." in: Journal of molecular neuroscience : MN, Vol. 64, Issue 1, pp. 129-139, 2018

Liu, Gao, Wang, Yang, Huo, Tian: "Effect of stromal cell-derived factor-1 on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction." in: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3282-3286, 2016

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN6269449) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) IHC analysis of TLR4 in mouse kidney tissue
Western Blotting (WB) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) Western blot analysis of TLR4 expression in Hela whole cell lysates,The lane on the l...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) ABIN6269450 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and perme...