anti-Rat (Rattus) TLR4 antibody for Immunofluorescence

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Epitope
pSer800
59
30
28
25
16
15
15
15
14
13
11
9
8
8
7
6
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
555
273
172
94
72
72
64
26
12
9
4
2
1
1
Host
Rabbit
338
273
47
8
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
46
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
393
341
186
137
129
123
98
92
34
32
27
26
26
21
20
16
14
8
7
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN6270322
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.5 ABIN6269449 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 3
4 ABIN6181202 FACS IF IHC (f) APC Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181209 FACS IF IHC (f) PerCP Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181210 FACS IF IHC (f) PE Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181213 FACS IF IHC (f) Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181184 FACS IF IHC (f) CF®647 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181186 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181192 FACS IF IHC (f) CF®770 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181190 FACS IF IHC (f) CF®568 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181196 FACS IF IHC (f) CF®680R Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181200 FACS IF IHC (f) CF®594 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181178 FACS IF IHC (f) CF405M Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181180 FACS IF IHC (f) CF®640R Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181182 FACS IF IHC (f) CF®543 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181194 FACS IF IHC (f) CF®680 Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181198 FACS IF IHC (f) CF®488A Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181176 FACS IF IHC (f) CF®405S Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181188 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181211 FACS IF IHC (f) Mouse IgG2a, kappa Log in to see TLR4-230 0
1 ABIN6181203 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG2a, kappa Log in to see TLR4-230 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Epitope pSer800
(59), (30), (28), (25), (16), (15), (15), (15), (14), (13), (11), (9), (8), (8), (7), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(555), (273), (172), (94), (72), (72), (64), (26), (12), (9), (4), (2), (1), (1)
Host Rabbit
(338), (273), (47), (8)
Clonality
Polyclonal
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (46), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(393), (341), (186), (137), (129), (123), (98), (92), (34), (32), (27), (26), (26), (21), (20), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling Images
Specificity Phospho-TLR4( Ser800) Antibody detects endogenous levels of TLR4.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human TLR4 around the phosphorylation site of Ser800.
Isotype IgG
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background

Description: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634, PubMed:27022195). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed:16622205).

Gene: TLR4

Molecular Weight 100kDa
Gene ID 7099
UniProt O00206
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol..
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) ABIN6270323 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) ABIN6270323 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was form...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) ABIN6270323 at 1/100 staining human gastric cancer tissue sections by IHC-P. The tiss...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) ABIN6270323 at 1/100 staining human liver tissue sections by IHC-P. The tissue was fo...
Western Blotting (WB) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) Western blot analysis of extracts from Hela, using Phospho-TLR4(Ser800) Antibody.
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Toll-Like Receptor 4 (TLR4) (pSer800) antibody (ABIN6270322) ABIN6270323 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilize...