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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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19 | ABIN6270322 | ICC IF IHC WB | Rabbit | IgG | pSer800 | Log in to see | Polyclonal | 0 | ||
14.5 | ABIN6269449 | ELISA ICC IF IHC WB | Rabbit | IgG | Log in to see | Polyclonal | 3 | |||
12.657918 | ABIN4360167 | BP CyTOF FACS ICC IF IHC (p) Neut WB | Mouse | IgG2b kappa | Log in to see | 76B357-1 | 24 | |||
4 | ABIN6181202 | FACS IF IHC (f) | APC | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
4 | ABIN6181209 | FACS IF IHC (f) | PerCP | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
4 | ABIN6181210 | FACS IF IHC (f) | PE | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
4 | ABIN6181213 | FACS IF IHC (f) | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | |||
1 | ABIN6181180 | FACS IF IHC (f) | CF®640R | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181196 | FACS IF IHC (f) | CF®680R | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181184 | FACS IF IHC (f) | CF®647 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181188 | FACS IF IHC (f) | CF®660R | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181194 | FACS IF IHC (f) | CF®680 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181198 | FACS IF IHC (f) | CF®488A | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181176 | FACS IF IHC (f) | CF®405S | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181178 | FACS IF IHC (f) | CF405M | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181186 | FACS IF IHC (f) | CF®555 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181190 | FACS IF IHC (f) | CF®568 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181192 | FACS IF IHC (f) | CF®770 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181200 | FACS IF IHC (f) | CF®594 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 | ||
1 | ABIN6181182 | FACS IF IHC (f) | CF®543 | Mouse | IgG2a, kappa | Log in to see | TLR4-230 | 0 |
General |
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Antigen | Toll-Like Receptor 4 (TLR4) Antibodies |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This TLR4 antibody is un-conjugated Alternatives |
Application |
Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Western Blotting (WB)
Alternatives
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72 references available |
Supplier | Log in to see |
Product Details anti-TLR4 AntibodyTarget Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN252522) Images |
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Purification | Protein G purified |
Immunogen | This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564). |
Clone | 76B357-1 |
Isotype | IgG2b kappa |
Plasmids, Primers & others | |
Target Details TLR4Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN252522) Images back to top |
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Antigen | |
Alternative Name | TLR4 (TLR4 Antibody Abstract) |
Background | Gene Symbol: TLR4 |
Gene ID | 7099 |
UniProt | O00206 |
Pathways | TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades |
Application DetailsProduct Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN252522) Images back to top |
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Application Notes | Western Blot 1-3 μg/mL, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen 1:500, In vitro assay, Flow (Cell Surface), Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), CyTOF-readyFlow (Intracellular) use reported in literature (See Cohen et al, 2008), Immunofluorescence (See Nowicki et al, 2009), IHC-F (Nowicki et al, 2012), Flow (cell surface) use reported in scientific literature (PMID 23796194). Use in chromatin immunoprecipitation reported in scientific literature (PMID 21966468). Use In vitro reported in scientific literature (PMID 26446256). |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN252522) Images back to top |
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Format | Liquid |
Concentration | 1.0 mg/mL |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-TLR4 antibody (ABIN252522)Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top |
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Product cited in: |
Chen, Yan, Guo: "Inhibitory effect of Patrinia on BRL-3A cell apoptosis through the TLR4/PI3K/AKT/GSK3β and TLR4/P38/JNK signaling pathways." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5344-5349, 2018 Bhattacharjee, Keyel: "Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses." in: Scientific reports, Vol. 8, Issue 1, pp. 6458, 2018 Domon, Nagai, Maekawa, Oda, Yonezawa, Takeda, Hiyoshi, Tamura, Yamaguchi, Kawabata, Terao: "Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia." in: Frontiers in immunology, Vol. 9, pp. 732, 2018 Sun, Li, Ma, Zhang, Song, Lv, He: "Inhibiting High Mobility Group Box-1 Reduces Early Spinal Cord Edema and Attenuates Astrocyte Activation and Aquaporin-4 Expression after Spinal Cord Injury in Rats." in: Journal of neurotrauma, 2018 Bernardini, Zannoni, Bertocchi, Tubon, Fernandez, Forni: "Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cells." in: BMC complementary and alternative medicine, Vol. 18, Issue 1, pp. 194, 2018 Bidne, Kvidera, Ross, Baumgard, Keating: "Impact of repeated lipopolysaccharide administration on ovarian signaling during the follicular phase of the estrous cycle in post-pubertal pigs." in: Journal of animal science, 2018 Choi, Kang, Song, Kim, Chung, Ylaya, Kim, Koh, Chung, Kim, Hewitt: "Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 12161, 2018 Mukherjee, Hough, Chattopadhyay, Grijalva, OConnor, Meriwether, Wagner, Ntambi, Navab, Reddy, Fogelman: "Role of enterocyte stearoyl-Co-A desaturase-1 in LDLR-null mice." in: Journal of lipid research, Vol. 59, Issue 10, pp. 1818-1840, 2018 Tobita, Watanabe, Saito et al.: "Specific vaginal lactobacilli suppress the inflammation induced by lipopolysaccharide stimulation through downregulation of toll-like receptor 4 expression in human embryonic intestinal epithelial ..." in: Bioscience of microbiota, food and health, Vol. 36, Issue 1, pp. 39-44, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human). Azuma, Taniguchi, Nakamura, Nagira, Khine, Kiyama, Uegaki, Izawa, Harada: "Lipopolysaccharide promotes the development of murine endometriosis-like lesions via the nuclear factor-kappa B pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 77, Issue 4, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)). Nakano, Shimazawa, Ojino, Izawa, Takeuchi, Inoue, Tsuruma, Hara: "Toll-like receptor 4 inhibitor protects against retinal ganglion cell damage induced by optic nerve crush in mice." in: Journal of pharmacological sciences, Vol. 133, Issue 3, pp. 176-183, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Camps-Bossacoma, Pérez-Cano, Franch, Untersmayr, Castell: "Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats." in: The Journal of nutritional biochemistry, Vol. 42, pp. 182-193, 2017 Schöniger, Gräfe, Schoon: "Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium." in: Veterinary immunology and immunopathology, Vol. 185, pp. 7-13, 2017 Szasz, Wenceslau, Burgess, Nunes, Webb: "Toll-Like Receptor 4 Activation Contributes to Diabetic Bladder Dysfunction in a Murine Model of Type 1 Diabetes." in: Diabetes, Vol. 65, Issue 12, pp. 3754-3764, 2017 Liu, Wang, Cao, Wang, Liu, Gao, Hu, Yuan, Lin: "Up-regulated TLR4 in cardiomyocytes exacerbates heart failure after long-term myocardial infarction." in: Journal of cellular and molecular medicine, Vol. 19, Issue 12, pp. 2728-40, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence Mishima, Ishihara, Oka, Fukuba, Oshima, Sonoyama, Yamashita, Tada, Kusunoki, Moriyama, Yuki, Kawashima, Kinoshita: "Decreased Frequency of Intestinal Regulatory CD5+ B Cells in Colonic Inflammation." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146191, 2016 (Sample species: Mouse (Murine)). Further details: Flow Cytometry Okada, Arai, Itoh, Adachi, Hayashida, Nakase, Ikemoto: "CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells' immune functions." in: Journal of leukocyte biology, Vol. 100, Issue 5, pp. 1093-1104, 2016 Li, Bao, Leung, Ma, Zhang, Lu, Wang, Han: "Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour." in: Molecules (Basel, Switzerland), Vol. 21, Issue 6, 2016 Kuramochi, Izawa, Pervin, Bondoc, Kuwamura, Yamate: "The kinetics of damage-associated molecular patterns (DAMPs) and toll-like receptors during thioacetamide-induced acute liver injury in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 68, Issue 8, pp. 471-7, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting Burgueño, Barba, Eyre, Romero, Neunlist, Fernández: "TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 187, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence |
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