We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Toll-Like Receptor 9 (TLR9) Antibodies|
|Reactivity||Dog (Canine), Horse (Equine), Human, Mouse (Murine), Primate, Rat (Rattus) Alternatives|
|Conjugate||This TLR9 antibody is un-conjugated Alternatives|
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), In vitro Assay (in vitro), Simple Western (SimWes), Western Blotting (WB)
|120 references available|
|Supplier||Log in to see|
Product Details anti-TLR9 AntibodyTarget Details TLR9 Application Details Handling References for anti-TLR9 antibody (ABIN4360430) Images
|Purification||Protein G purified|
|Immunogen||This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).|
|Plasmids, Primers & others|
Target Details TLR9Product Details anti-TLR9 Antibody Application Details Handling References for anti-TLR9 antibody (ABIN4360430) Images back to top
|Alternative Name||TLR9 (TLR9 Antibody Abstract)|
|Background||Gene Symbol: TLR9|
|Pathways||TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-TLR9 Antibody Target Details TLR9 Handling References for anti-TLR9 antibody (ABIN4360430) Images back to top
|Application Notes||Western Blot 2-5 μg/mL, Simple Western 30 μg/mL, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 5 μg/mL, Functional, In vitro assay, Flow (Intracellular) 1:10 - 1:1000, CyTOF-readyStaining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple pieces of scientific literature Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078)|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TLR9 Antibody Target Details TLR9 Application Details References for anti-TLR9 antibody (ABIN4360430) Images back to top
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-TLR9 antibody (ABIN4360430)Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Handling Images back to top
|Product cited in:||
Leppänen, Helminen, Huhta, Kauppila, Isohookana, Haapasaari, Lehenkari, Saarnio, Karttunen: "High toll-like receptor (TLR) 9 expression is associated with better prognosis in surgically treated pancreatic cancer patients." in: Virchows Archiv : an international journal of pathology, Vol. 470, Issue 4, pp. 401-410, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).
Heftrig, Sturm, Oppermann, Kontradowitz, Jurida, Schimunek, Woschek, Marzi, Relja: "Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients." in: Mediators of inflammation, Vol. 2017, pp. 2608349, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Huhta, Helminen, Kauppila, Salo, Porvari, Saarnio, Lehenkari, Karttunen: "The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 64, Issue 8, pp. 470-82, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).
Sun, Xiao, Luo, Zhao, Pu, Zhao, Chen, Wang, Liao: "High-glucose induces tau hyperphosphorylation through activation of TLR9-P38MAPK pathway." in: Experimental cell research, Vol. 359, Issue 2, pp. 312-318, 2017 Method employed by authors: Western Blotting (WB)
Chandler, Keene, Tuomela, Forero-Torres, Desmond, Vuopala, Harris, Merner, Selander: "Lower frequency of TLR9 variant associated with protection from breast cancer among African Americans." in: PLoS ONE, Vol. 12, Issue 9, pp. e0183832, 2017 (Sample species: Human).
Duhamel, Rodet, Murgoci, Desjardins, Gagnon, Wisztorski, Fournier, Day, Salzet: "The proprotein convertase PC1/3 regulates TLR9 trafficking and the associated signaling pathways." in: Scientific reports, Vol. 6, pp. 19360, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Siponen, Bitu, Al-Samadi, Nieminen, Salo: "Cathepsin K expression is increased in oral lichen planus." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 45, Issue 10, pp. 758-765, 2016
Schnerch, Prasse, Vlachakis, Schuchardt, Pechkovsky, Goldmann, Gaede, Müller-Quernheim, Zissel: "Functional Toll-Like Receptor 9 Expression and CXCR3 Ligand Release in Pulmonary Sarcoidosis." in: American journal of respiratory cell and molecular biology, Vol. 55, Issue 5, pp. 749-757, 2016
Meseure, Vacher, Drak Alsibai, Trassard, Nicolas, Leclere, Lerebours, Guinebretiere, Marangoni, Lidereau, Bieche: "Biopathological Significance of TLR9 Expression in Cancer Cells and Tumor Microenvironment Across Invasive Breast Carcinomas Subtypes." in: Cancer microenvironment : official journal of the International Cancer Microenvironment Society, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Mishima, Ishihara, Oka, Fukuba, Oshima, Sonoyama, Yamashita, Tada, Kusunoki, Moriyama, Yuki, Kawashima, Kinoshita: "Decreased Frequency of Intestinal Regulatory CD5+ B Cells in Colonic Inflammation." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146191, 2016 (Sample species: Mouse (Murine)). Further details: Flow Cytometry
Acharya, Sokolovska, Tam, Conway, Stefani, Raso, Mukhopadhyay, Feliu, Paul, Savill, Hynes, Xavier, Vyas, Stuart, Lacy-Hulbert: "αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells." in: Nature communications, Vol. 7, pp. 10917, 2016 (Sample species: Mouse (Murine)). Further details: Flow Cytometry
Burgueño, Barba, Eyre, Romero, Neunlist, Fernández: "TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 187, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence
Mu, Wang, Wang, Zhang, Shang, Tan, Li, Chen: "Tumor Location and Survival Outcomes in Adult Patients with Supratentorial Glioblastoma by Levels of Toll-Like Receptor 9 Expression." in: World neurosurgery, Vol. 97, pp. 279-283, 2016
Moreira, Costa-Pereira, Alves, Marteleto, Ribeiro, Peruhype-Magalhães, Giunchetti, Martins-Filho, Araújo: "Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes." in: Veterinary parasitology, Vol. 220, pp. 33-45, 2016 Method employed by authors: Flow Cytometry (FACS)
Jeon, Kim, Koh, Chung, Ha: "Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination." in: Oncotarget, Vol. 7, Issue 27, pp. 41811-41824, 2016 Method employed by authors: Dot Blot (DB) (Sample species: Human).
Dillmann, Ringel, Ringleb, Mora, Olesch, Fink, Roberts, Brüne, Weigert: "S1PR4 Signaling Attenuates ILT 7 Internalization To Limit IFN-α Production by Human Plasmacytoid Dendritic Cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1579-90, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Helminen, Huhta, Kauppila, Lehenkari, Saarnio, Karttunen: "Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 125, Issue 2, pp. 85-92, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).
Evangelista, Castro, Alves, Dias, Souza, Reis, Silva, Castañon, Farias, Juliano, Ferreira: "Early IFN-γ production together with decreased expression of TLR3 and TLR9 characterizes EAE development conditional on the presence of myelin." in: Autoimmunity, Vol. 49, Issue 4, pp. 258-67, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).
Lim, Kim, Nguyen, Cho: "The role of TLR9 in stress-dependent autophagy formation." in: Biochemical and biophysical research communications, Vol. 481, Issue 3-4, pp. 219-226, 2016 (Sample species: Human).
Kauppila, Korvala, Siirilä, Manni, Mäkinen, Hagström, Atula, Haglund, Selander, Saarnio, Karttunen, Lehenkari, Salo: "Toll-like receptor 9 mediates invasion and predicts prognosis in squamous cell carcinoma of the mobile tongue." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 44, Issue 8, pp. 571-7, 2015 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections),Blocking Peptide,Neutralization
ImagesProduct Details anti-TLR9 Antibody Target Details TLR9 Application Details Handling References for anti-TLR9 antibody (ABIN4360430) back to top