anti-Horse (Equine) TLR9 antibody for Western Blotting

Recommended TLR9 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 9 (TLR9) Antibodies
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
Epitope
AA 268-284
39
32
15
12
12
11
8
7
7
6
6
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
262
147
55
35
22
20
6
5
3
2
2
2
2
2
1
1
1
Host
Mouse
144
123
35
14
4
3
Clonality
Monoclonal
Conjugate
This TLR9 antibody is conjugated to FITC
27
24
17
8
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
228
162
131
109
88
38
37
36
14
7
6
6
6
5
5
3
3
3
2
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Catalog No. ABIN364233
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4.8026066 ABIN2777826 WB Rabbit N-Term Log in to see Polyclonal 1
4 ABIN467852 WB Rabbit AA 251-300 Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 9 (TLR9) Antibodies
Epitope AA 268-284
(39), (32), (15), (12), (12), (11), (8), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
(262), (147), (55), (35), (22), (20), (6), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(144), (123), (35), (14), (4), (3)
Clonality
Monoclonal
Conjugate This TLR9 antibody is conjugated to FITC
(27), (24), (17), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(228), (162), (131), (109), (88), (38), (37), (36), (14), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2)
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Product Details anti-TLR9 Antibody

Target Details TLR9 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.
Purification Protein G purified
Immunogen Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).

Type of Immunogen: Synthetic peptide
Isotype IgG1
Plasmids, Primers & others

Target Details TLR9

Product Details anti-TLR9 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TLR9 (TLR9 Antibody Abstract)
Background Name/Gene ID: TLR9
Family: Toll-like Receptor

Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9
Gene ID 54106
UniProt Q9NR96
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR9 Antibody Target Details TLR9 Handling Images back to top
Application Notes Approved: Flo, ICC, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry (Intracellular): 0.1-2 μg/10^6 cells, cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM Tris, 150 mM sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles. Sensitive to light.
Storage 4 °C,-20 °C
Storage Comment Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Images

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 9 (TLR9) (AA 268-284) antibody (FITC) (ABIN364233) Human Spleen (formalin-fixed, paraffin-embedded) stained with TLR9 antibody ABIN36423...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 9 (TLR9) (AA 268-284) antibody (FITC) (ABIN364233) Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraf...