anti-Human TLR9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TLR9 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 9 (TLR9) Antibodies
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
Reactivity
Human
261
148
55
36
23
20
7
5
3
2
2
2
2
2
1
1
Host
Mouse
143
123
35
14
4
3
Clonality
Monoclonal
Conjugate
This TLR9 antibody is conjugated to Biotin
26
25
17
8
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
227
162
132
109
88
39
37
36
14
7
6
6
6
5
5
3
3
3
2
Options
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Catalog No. ABIN2191964
$ 405.93
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.57224 ABIN2191963 FACS IF IHC (p) WB FITC Mouse IgG2a Log in to see 5G5 8
27.57224 ABIN2191962 FACS IF IHC (p) WB Mouse IgG2a Log in to see 5G5 8
21.57224 ABIN2854849 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
11.5 ABIN749588 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal 1
11.120814 ABIN337069 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1, kappa AA 268-284 Log in to see 26C593-2 0
11.120814 ABIN364233 FACS ICC IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 AA 268-284 Log in to see 0
11.120814 ABIN214575 IHC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
11.120814 ABIN214619 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
11.120814 ABIN951280 IHC (p) WB Rabbit IgG AA 26-300 Log in to see Polyclonal 0
10 ABIN1104704 FACS IHC IHC (p) WB Rat IgG2a, kappa Log in to see 72-1665 0
10 ABIN3026222 FACS IHC (p) WB Mouse IgG1, kappa N-Term Log in to see TR9541 0
8.5 ABIN2479764 IHC (p) WB Goat IgG N-Term Log in to see Polyclonal 1
8.120814 ABIN2744930 IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.120814 ABIN784145 EIA IF IHC (p) WB Goat IgG AA 204-224, N-Term Log in to see Polyclonal 0
7 ABIN790626 IHC IHC (p) WB Rabbit AA 26-300 Log in to see Polyclonal 0
7 ABIN1724138 FACS IHC (p) WB Rat IgG2a kappa Log in to see 72-1665 0
7 ABIN5589572 FACS IHC (p) WB Rat IgG2a kappa Log in to see 72-1665 0
5.5 ABIN2479766 IHC (fro) FACS IHC (p) WB Mouse IgG2a Log in to see 5G5 3
4 ABIN1106226 EIA IHC (fro) IF IHC (p) WB FITC Mouse IgG2a AA 1-815, Extracellular Domain, N-Term Log in to see 5G5 7
4 ABIN1106225 EIA IHC (fro) IF IHC (p) WB Biotin Mouse IgG2a AA 1-815, Extracellular Domain, N-Term Log in to see 5G5 7

General

Antigen Toll-Like Receptor 9 (TLR9) Antibodies
Reactivity Human
(261), (148), (55), (36), (23), (20), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(143), (123), (35), (14), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR9 antibody is conjugated to Biotin
(26), (25), (17), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(227), (162), (132), (109), (88), (39), (37), (36), (14), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2)
Pubmed 8 references available
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Product Details anti-TLR9 Antibody

Target Details TLR9 Application Details Handling References for anti-TLR9 antibody (ABIN2191964) Images
Cross-Reactivity (Details) Cross reactivity: Canine TLR9 : Yes, Mouse TLR9 : Yes
Sterility 0.2 μm filtered
Clone 5G5
Isotype IgG2a
Plasmids, Primers & others

Target Details TLR9

Product Details anti-TLR9 Antibody Application Details Handling References for anti-TLR9 antibody (ABIN2191964) Images back to top
Antigen
Alternative Name Toll-Like Receptor 9 (TLR9 Antibody Abstract)
Background The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN- regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens. Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles. Aliases CD289, TLR9 Immunogen Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR9 Antibody Target Details TLR9 Handling References for anti-TLR9 antibody (ABIN2191964) Images back to top
Application Notes For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive RAW264.7 macrophages stimulated with IFNγ control
Restrictions For Research Use only

Handling

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details References for anti-TLR9 antibody (ABIN2191964) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TLR9 antibody (ABIN2191964)

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Handling Images back to top
Product cited in:

Machida, Ito, Hirose, Takeshita, Oshiro, Nakamura, Mori, Inayama, Yan, Kobayashi, Yokota: "Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2530-537, 2010

Zheng, Wang, Liu, Liu, Feng, Wu, Cao: "Distinct host-related dendritic cell responses during the early stage of Plasmodium yoelii infection in susceptible and resistant mice." in: Parasite immunology, Vol. 32, Issue 5, pp. 324-34, 2010

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Tokumasa, Suto, Kagami, Furuta, Hirose, Watanabe, Saito, Shimoda, Iwamoto, Nakajima: "Expression of Tyk2 in dendritic cells is required for IL-12, IL-23, and IFN-gamma production and the induction of Th1 cell differentiation." in: Blood, Vol. 110, Issue 2, pp. 553-60, 2007

Pratesi, Petrangolini, Tortoreto, Addis, Belluco, Rossini, Selleri, Rumio, Menard, Balsari: "Therapeutic synergism of gemcitabine and CpG-oligodeoxynucleotides in an orthotopic human pancreatic carcinoma xenograft." in: Cancer research, Vol. 65, Issue 14, pp. 6388-93, 2005

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004

Rumio, Besusso, Palazzo, Selleri, Sfondrini, Dubini, Ménard, Balsari: "Degranulation of paneth cells via toll-like receptor 9." in: The American journal of pathology, Vol. 165, Issue 2, pp. 373-81, 2004

Background publications

Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002

Images

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Handling References for anti-TLR9 antibody (ABIN2191964) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 9 (TLR9) antibody (Biotin) (ABIN2191964) THP1 cells were incubated with IgG2a isptype controle (A) or α-TLR9 5G5 mAb (B). Cell...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 9 (TLR9) antibody (Biotin) (ABIN2191964) THP1 cells were incubated with IgG2a isptype controle (A) or α-TLR9 5G5 mAb (B). Cell...