anti-Human Cytoplasmic Protein NCK1 antibody for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Reactivity
Human, Monkey
138
39
29
6
3
3
3
3
2
2
2
2
2
1
1
1
Host
Mouse
92
45
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
136
77
55
15
14
11
8
6
6
2
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN969545
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.285944 ABIN1774727 ICC WB Mouse IgG1 Log in to see EM-06 1
19.712444 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
16.712444 ABIN393431 ELISA WB Mouse IgG1 kappa AA 185-294 Log in to see 1A1 5
16.712444 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
16.712444 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
15.785943 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal 0
15.785943 ABIN5663589 IF IHC WB Rabbit Log in to see Polyclonal 0
13.712444 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.712444 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294 Log in to see 1A1 0
12.073499 ABIN6388766 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.073499 ABIN5621931 WB Rabbit IgG Log in to see Polyclonal 0
12.073499 ABIN2140080 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see AT6B3 0
12.073499 ABIN2147272 ELISA WB Mouse IgG1 kappa Log in to see 11D10 0
12.073499 ABIN2147273 IP WB Mouse IgG2a lambda Log in to see AF6D7 0
12.073499 ABIN2147274 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.073499 ABIN6144405 WB Rabbit Log in to see Polyclonal 0
10.712444 ABIN518212 IF WB Mouse AA 1-377 Log in to see Polyclonal 0
10.712444 ABIN1327536 IF WB Mouse AA 1-377 Log in to see Polyclonal 0
10.712444 ABIN5014021 ICC IHC WB Rabbit IgG AA 2-377 Log in to see Polyclonal 0
10.712444 ABIN2917490 IF/ICC IHC IP WB Rabbit AA 2-377 Log in to see Polyclonal 0

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Reactivity Human, Monkey
(138), (39), (29), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(92), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(136), (77), (55), (15), (14), (11), (8), (6), (6), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images
Purification Purified
Immunogen Purified recombinant fragment of human NCK1 expressed in E. Coli.
Clone 5B7
Isotype IgG1
Plasmids, Primers & others

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Molecular Weight 47 kDa
Gene ID 4690
HGNC 4690
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Product cited in:

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, 2008

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) back to top
Supplier Images
ELISA image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 2: Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and n...
Immunohistochemistry (IHC) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 4: Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HE...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 5: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recom...