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|Antigen||Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This CAMK2A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CAMK2A AntibodyTarget Details CAMK2A Application Details Handling Images
|Cross-Reactivity (Details)||Species reactivity (tested):Rat, Human.|
|Characteristics||Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha|
|Purification||Protein G Chromatography.|
Target Details CAMK2AProduct Details anti-CAMK2A Antibody Application Details Handling Images back to top
|Alternative Name||CAMK2A (CAMK2A Antibody Abstract)|
|Background||CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, KIAA0968|
|Pathways||WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction|
Application DetailsProduct Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
|Application Notes||ELISA. Immunflourescence: 10 μg/mL. Immunoprecipitaion. ImmunohistochemistryWestern blot: 1 μg/mL, A band of approx. 50 kDa is detected.1 μg/mL of CAMKII was sufficient for detection of 0.2 μg CAMKII by colorimetric immunoblot. (Ref.13)|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
|Buffer||PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|