anti-Human CAMK2A antibody for Western Blotting

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin dependent protein kinase II alpha
  • calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2A
  • camk2a.S
  • camk2a
  • Camk2a
Epitope
Internal Region
27
27
13
11
10
9
6
6
6
5
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
143
93
92
26
10
6
5
4
3
3
2
1
1
1
1
1
1
1
1
Host
Goat
116
58
18
Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
7
7
7
6
6
6
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
188
96
72
55
46
36
27
10
7
6
4
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN570984
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN391311 IHC (p) WB Rabbit Ig Fraction AA 446-478, C-Term Log in to see Polyclonal 1
3.1357021 ABIN393580 ELISA IF WB Mouse IgG2b kappa AA 305-410 Log in to see 2C12 0
3.1357021 ABIN393584 ELISA IF WB Mouse IgG2a kappa AA 305-410 Log in to see 2C4 0
3.1357021 ABIN391617 IHC (p) WB Rabbit Ig Fraction AA 344-371, C-Term Log in to see Polyclonal 0
3.1357021 ABIN359578 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 2
3.1357021 ABIN359400 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 1
3.1357021 ABIN6260429 ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN1870029 WB Rabbit IgG pThr286 Log in to see Polyclonal 0
3.1357021 ABIN498777 IHC (p) WB Rabbit pThr286 Log in to see Polyclonal 0
3.1357021 ABIN513972 IF ELISA WB Mouse IgG2b kappa AA 305-410, partial Log in to see 2C12 0
3.1357021 ABIN513970 IF ELISA WB Mouse IgG2a kappa AA 305-410, partial Log in to see 2C4 0
3.1357021 ABIN498462 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1357021 ABIN498753 IF IHC (p) WB Rabbit pThr286 Log in to see Polyclonal 0
3.1357021 ABIN498868 IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1357021 ABIN2464145 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.1357021 ABIN129648 ELISA WB Rabbit IgG AA 6-23 Log in to see Polyclonal 0
3.1357021 ABIN1871411 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN2987742 WB Rabbit IgG pThr286 Log in to see Polyclonal 0
3.1357021 ABIN5573662 IHC (p) WB Rabbit pThr305 Log in to see Polyclonal 0
3.1357021 ABIN5573664 ELISA IF IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope Internal Region
(27), (27), (13), (11), (10), (9), (6), (6), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(143), (93), (92), (26), (10), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(116), (58), (18)
Clonality
Polyclonal
Conjugate This CAMK2A antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(188), (96), (72), (55), (46), (36), (27), (10), (7), (6), (4), (2), (2), (1)
Supplier Log in to see

Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling Images
Purpose CAMK2A
Blocking Peptide Blocking peptide for this product available: ABIN2815316
Sequence PRTAQSEETR VWHR
Specificity This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
Plasmids, Primers & others

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain
Gene ID 815
NCBI Accession NP_057065, NP_741960
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
Application Notes

DS WB Results: Approx 58-60 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3 kDa according to Human NP_057065.2, and 54.1 kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50 kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3 μg/mL. Primary incubation 1 hour at room temperature.

DS IHC Results: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment

Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody (ABIN570984) Antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lys...