anti-Human CAMK2A antibody for Western Blotting

Recommended CAMK2A Antibody

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin dependent protein kinase II alpha
  • calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2A
  • camk2a.S
  • camk2a
  • Camk2a
Epitope
Internal Region
24
22
12
10
9
7
6
6
5
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
150
93
88
25
6
6
4
4
3
2
2
1
1
1
1
1
1
1
1
Host
Goat
122
53
19
Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
9
7
7
6
6
6
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
178
93
69
51
42
34
26
8
7
5
3
2
2
1
Options

Available images

Catalog No. ABIN570984
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
17.559069 ABIN129648 ELISA WB Rabbit IgG AA 6-23 Polyclonal 0
17.559069 ABIN5663715 WB Rabbit Polyclonal 0
16.857681 ABIN513970 IF ELISA WB Mouse IgG2a kappa AA 305-410 2C4 0
16.857681 ABIN513972 IF ELISA WB Mouse IgG2b kappa AA 305-410 2C12 0
16 ABIN513973 IF PLA ELISA WB Mouse IgG2b kappa AA 305-410 2C11 0
13.701386 ABIN5619026 ICC IF IHC WB Rabbit Center Polyclonal 0
13.701386 ABIN2135323 ELISA WB Rabbit IgG Polyclonal 0
13.701386 ABIN3206823 WB Rabbit IgG pThr286 Polyclonal 0
12.357681 ABIN543732 IHC (p) ELISA WB Rabbit Polyclonal 3
10.857681 ABIN359400 EIA IHC (p) WB Rabbit Ig Fraction C-Term Polyclonal 1
10.857681 ABIN5573664 ELISA IF IHC (p) WB Rabbit Polyclonal 0
10.857681 ABIN3003199 IHC WB Rabbit IgG Polyclonal 0
10.857681 ABIN3003046 IHC WB Rabbit IgG Polyclonal 0
10 ABIN574315 ELISA IHC IHC (p) WB Goat AA 455-468 Polyclonal 0
10 ABIN5533833 IHC (p) WB Rabbit Ig Fraction AA 446-478, C-Term Polyclonal 0
10 ABIN5533834 IHC (p) WB Rabbit Ig Fraction AA 344-371, C-Term Polyclonal 0
9.357681 ABIN5693074 ELISA WB Rabbit AA 392-457 Polyclonal 1
9.357681 ABIN550192 WB Rabbit Polyclonal 3
7.8576813 ABIN359578 EIA IHC (p) WB Rabbit Ig Fraction C-Term Polyclonal 2
7.8576813 ABIN498777 IHC (p) WB Rabbit pThr286 Polyclonal 0

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope Internal Region
(24), (22), (12), (10), (9), (7), (6), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(150), (93), (88), (25), (6), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(122), (53), (19)
Clonality
Polyclonal
Conjugate This CAMK2A antibody is un-conjugated
(9), (7), (7), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(178), (93), (69), (51), (42), (34), (26), (8), (7), (5), (3), (2), (2), (1)
Supplier

Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling Images
Purpose CAMK2A
Blocking Peptide Blocking peptide for this product available: ABIN2815316
Sequence PRTAQSEETR VWHR
Specificity This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
Plasmids, Primers & others

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain
Gene ID 815
NCBI Accession NP_057065, NP_741960
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
Application Notes

DS WB Results: Approx 58-60 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3 kDa according to Human NP_057065.2, and 54.1 kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50 kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3 μg/mL. Primary incubation 1 hour at room temperature.

DS IHC Results: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment

Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody (ABIN570984) Antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lys...